210 METALLIC STAINS (IMPREGNATION METHODS). 



POL fixes in perchloride ( 80) and treats for twenty-four hours with 

 alcohol containing a trace of gallic acid. 



POLAILLON (loc. cit.) reduces in tannic acid. 



The method is not applicable to chromic objects. 



GOLODETZ and UNNA (Monats. prakt. Derm., xlviii, 1909, p. 153) 

 put sections of skin for five minutes into fresh mixture of 1 per cent, 

 perchloride of iron and 1 per cent. sol. of ferricyanide of potassium. 

 See also UNNA and GOLODETZ, ibid., xlix, 1909, p. 97. 



ROOSEVELT ( Med. Rec., ii, 1887, p. 84 ; Journ. Eoy. Mic. Soc., 1888, 

 p. 157} employs a stain composed of 20 drops of saturated solution of 

 iron sulphate, 30 grms. water, and 15 to 20 drops pyrogallic acid. 



376. Palladium Chloride (see SCHULZE, 77). Prussian Blue (see 

 LEBER, Arch. Ophthalm., xiv, p. 300 ; RANVIER, Traite, p. 108). Cuprie 

 Sulphate (see LEBER, ibid.). Lead Chromate (see LEBER, ibid.). 

 Sulphides (see LANDOIS, Centralb. med. Wiss., 1885, No. 55 ; and 

 GIERKE, in Zeit. wiss. Mile., i, 1884, p. 497). Molybdate of Ammonia 

 (MERKEL ; KRAUSE) (see GIERKE, ibid., i, 1884, p. 96). Oxychloride of 

 Ruthenium (NICOLLE and CANTACUZENE) (see Ann. Inst. Pasteur, vii, 

 1893, p. 331). Ruthenium Red (Ruthenium Sesquichloride) (EiSEN, 

 Zeit. wiss. Mik., xiv, 1897, p. 200 ; in my hands totally useless). Oxide 

 of Manganese (GOLODETZ and UNNA, Monats. prakt. Derm., xlviii, 

 1909, p. 151). 



