CHAPTER XVIII. 215 



240 c.c. (from SQUIRE'S Methods and Formula, p. 42). Using 

 orange G (not mentioned by SQUIRE), I have had very good results. 

 The method of CAVAZZANI (Riforma Med., Napoli, 1893, p. 604 ; 

 Zeit. wiss. Mik., xi, 3, 1894, p. 344) is far too complicated. 



398. Haematoxylin and Picro-Saurefuchsin (VAN GIESON, New 

 York Med. Journ., 1889, p. 57 ; quoted from MOELLER, Zeit. wiss. 

 Mik., xv, 2, 1898, p. 172, which see for further details). Proceed 

 as above, using for the second stain the picro-Saurefuchsin mixture, 

 299. The second stain must not be too prolonged. 



WEIGERT (Zeit. wiss. Mik., xxi, 1904, p. 1) stains first in his iron- 

 haematoxylin mixture ( 244), rinses in water, and stains for a short 

 time in his picro-Saurefuchsin ( 299), rinses, dehydrates with 

 90 per cent, alcohol, and clears with carbolic acid-xylol mixture 

 ( 167). 



