CHAPTER XXVII. 341 



to four minutes in concentrated aqueous solution of Kresykchtviolett, 

 differentiates in alcohol, clears in xylol, and mounts in balsam. 



See also UNNA, Monatsschr. prakt. Derm., xx, 1895, p. 69 ; the article 

 " Haut " in the Encycl. mik. Technik. ; and UNNA and GOLODETZ, 

 Monatsschr. prakt. Derm., xlix, 1909, p. 95 ; LAFFONT, Eibl. Anat., 1909, 

 p. 209. 



For Triehohyalin, see GAVAZZENI, Monatsschr. prakt. Derm., xlvii, 

 1908, p. 229. 



718. Eleidin. To demonstrate the stratum granulosum and the 

 eleidin granules RANVIER (Arch. Anat. Micr., iii, 1899, p. 1) hardens 

 with alcohol, stains with picro-carmine, and treats with lime-water. 

 The cells swell and show up the granules, which do not change. 

 See loc. cit., other methods for the study of skin. 



Buzzi (see Encycl. mik. Technik., article " Haut ") stains sections 

 for a few minutes in a watch-glassful of water with 2 to 3 drops of 

 1 per cent. Congo red. Similarly WEIDENREICH, Arch. mik. Anat., 

 Ivii, 1901, p. 583. Other authors recommend nigrosin, or Wasser- 

 blau, or orcein. 



See also JOSEPH, " Dermatohist. Technik," Berlin, 1905, and 

 DREUW, Med. Klinik, Berlin, 1907, Nos. 27 and 28. 



For Cholesterin see GOLODETZ and UNNA, Monatsschr. prakt. 

 Derm., xlvii, 1908, p. 1. 



719. Horn, Hair, and Nails. The elements of hairs and nails may 

 be isolated by prolonged maceration in 40 per cent, potash solution, 

 or by heating with concentrated sulphuric acid. See also VON 

 NATHUSIUS, Zool. Anz., xv, 1892, p. 395. 



Horny tissues stain well in safranin or gentian violet (REINKE, 

 Arch. f. mik. Anat., xxx, 1887, p. 183 ; ERNST, ibid., xlvii, 1896, 

 p. 669 ; RABL, ibid., xlviii, 1896, p. 489). 



UNNA (op. cit. last section, p. 598) stains the tyrosin-bearing 

 keratin in sections of skin for a few seconds or minutes in a mixture 

 of 5 parts of Millon's reagent, 5 of water, and 1 of glycerin, treats 

 shortly with nitric acid of 25 per cent., and mounts in balsam. 



720. Skin-nerves and Nerve-endings. Impregnate with gold 

 chloride. See Chapter XVII., especially 365. 



721. Tactile Corpuscles.- See 364 366. Gold methods are 

 indicated. See also RANVIER, Traite, p. 919 ; LANGERHANS, Arch, 

 mik. Anat., 1873, p. 730 ; KULTSCHIZKY, ibid., 1884, p. 358 ; and 

 SMIRNOW, Intern. Monatsschr. f. Anat., etc., x, 1893, p. 241, who 

 recommends, besides the gold method of Lowit, the rapid bichromate 

 of silver method of Golgi. 



