342 TEGUMENTARY ORGANS. 



Corpuscles of Herbst and Corpuscles of Grandry. DOGIEL 

 (Arch. Anat. u. EntwickeL, 1891, p. 182) has used the methylen 

 blue method. Four per cent, solution of methylen blue, warmed to 

 40 C., is injected into blood-vessels of the heads of ducks or geese ; 

 pieces of skin are removed from the beaks, sectioned in pith, and 

 the sections brought on to slides and moistened with aqueous or 

 vitreous humour from the animal and left for ten to thirty minutes 

 exposed to the air, then brought into picrate of ammonia, and treated 

 as described 343. GEBERG (Intern. Monatsschr. Anat., x, 1893, 

 p. 205) made use of a method of ARNSTEIN, according to which 

 pieces of skin are put for twenty-four hours into lime-water, the horny 

 layer removed, the pieces treated for five minutes with 0-25 per- 

 cent. gold chloride, reduced in water, and the precipitate that 

 forms on them removed by putting into O25 per cent, cyanide of 

 potassium and brushing. 



NOWAK (Anat. Anz., xxxvi, 1910, p. 217) takes UNNA'S Orce'in- 

 wasserblau mixture (Wasserblau O.D., 1 part, orcei'n 1, acetic acid 5, 

 glycerin 20, alcohol 50, water 100) and adds to it 1 part more of 

 orcein. To 10 c.c. of this he adds at the moment of using 10 c.c. 

 of 1 per cent, solution of eosin in alcohol of 80 per cent, and 3 c.c. 

 of 1 per cent, solution of hydroquinon. Stain for five to ten minutes. 

 rinse, stain for ten minutes in 1 per cent, aqueous solution of 

 safranin, wash, treat for thirty minutes with 0-5 per cent, solution 

 of bichromate of potash, dehydrate and mount. 



Similarly DOGIEL, Folia NeurobioL, iv, 1910, p. 218 (also employing 

 Bielschowsky's neurofibril method). 



723. Corpuscles of Meissner and of Krause (Cornea and Conjunc- 



tiva). DOGIEL (Arch.'f. mik. Anat., xxxvii, 1891, p. 602, and xliv, 

 1894, p. 15) employs the methylen blue method ; for details see 

 previous editions. 



See also LONGWORTH'S methods, Arch. mik. Anat., 1875, p. 655. 



724. Similar Objects. Papillae Foliatae of the Rabbit, HERMANN, see 

 Zeit. wiss. Mik., v, 1888, p. 524 ; ARNSTEIN, ibid., xiii, 1897, p. 240. 

 Olf active Organs of Vertebrates, DOGIEL, Arch. mile. Anat., 1887, p. 74. 

 Organs of a " Sixth Sense " in Amphibia, MITROPHANOW, Zeit. wiss. Mik., 

 v, 1888, p. 513 (details as to staining with " Wasserblau," for which sec 

 also Biol. Centralb., vii, 1887, p. 175). Nerve-endings in Tongue of Frog, 

 FAJERSTAIN, Arch, de Zool. exper. et gen., vii, 1889, p. 705. Tongue of 

 Rabbit, VON LENHOSSEK, Zeit. wiss. Mik., xi, 1894, p. 377 (Ramon y 

 Cajal's double Golgi methpd). 



725. Cornea. There are three chief methods- the methylen blue, 

 the silver, and the gold method. 



