CHAPTER XXVIII. 



MUSCLE AND TENDON (NERVE-ENDINGS ). 



Striated Muscle. 



727. Muscle-cells. For these and allied subjects see, inter alia, 

 BEHRENS, KOSSEL, und SCHIEFFERDECKER, Das Mikroshop, etc., 

 vol. ii, pp. 154 161 ; and SCHAFER, Proc. Roy. Soc., xlix, 1891, 

 p. 280. 



Iron hsematoxylin gives very fine images of striped muscle, and 

 so does Mallory's phospho-tungstic. 



For dissociation methods see 512 545. 



To isolate the sarcolemma SOLGER (Zeit. wiss. Mik., vi, 1889, 

 p. 189) teases fresh muscle in saturated solution of ammonium 

 carbonate. 



728. Nerve-endings the Methylen Blue Method. For BIEDER- 



M ANN'S procedure for the muscles of Astacus see 342 (see also 

 Zeit. wiss. Mik., vi, 1889, p. 65). After impregnating as there 

 directed the carapace should be opened, and the muscles exposed 

 to the air in a roomy moist chamber for from two to six hours. 



For Hydrophilus piceus, BIEDERMANN proceeded by injecting 

 0-5 c.c. of methylen blue solution between the ultimate and pen- 

 ultimate abdominal rings, in the ventral furrow, and keeping the 

 animals alive in water for three to four hours, then opened the 

 thorax by two lateral incisions, and removed the muscles of the 

 first pair of legs and exposed them to the air for three or four hours 

 in a moist chamber, and finally examined in salt solution. 



GERLACH (Sitzb. Akad. Wiss. Munchen, 1889, ii, p. 125) injected 

 frogs, either through the abdominal vein or through the aorta, 

 with 4 to 5 c.c. of a 1 : 400 solution in 1 per cent, salt solution, and 

 examined pieces of muscle in serum of the animal, afterwards fixing 

 with picrate of ammonia and mounting in glycerin jelly. 



The procedure of DOGIEL has been given, 342. 



729. Nerve-endings the Gold Method. FISCHER (Arch. mik. 

 Anat., 1876, p. 365) used the method of Lowrr. 



BIEDERMANN (last section) recommends for Astacus a similar 



