CHAPTER XXV III. 347 



Tendon. 



734. Tendons. RETTERER (C. R. Soc. BioL, x, 1898, p. 580) 

 fixes in equal parts of saturated solutions of sublimate and picric 

 acid, puts for one to three days into saturated picric acid with 

 2 to 3 per cent, of sodium chloride, to remove the mucin, and imbeds 

 in paraffin. 



735. Union of Muscle and Tendon. For this see RETTERER and 

 LELIEVRE, C. R. Soc. Biol., 1911, No. 12 (orcei'n for twenty-four 

 hours, followed by iron haematoxylin) ; and SCHULTZE (Verh. phys. 

 med. Ges. Wilrzburg, 1911, p. 33) (treats for a day or two with a 

 mixture of equal parts of 2 per cent, bichromate of potash and 

 alcohol, in the dark, then for two days with 0-5 per cent, solution of 

 hsematoxylin in alcohol of 70 per cent., then with Van Gieson's 

 picro-sauref uchsin) . 



736. Corpuscles of Golgi (RANVIER, Traite, p. 929). Take the 

 tendon of the anterior and superior insertion of the gemini muscles 

 of the rabbit. Treat it by the formic acid and gold method ( 365), 

 and after reduction scrape with a scalpel, in order to remove the 

 muscle-fibres that mask the musculo-tendinous organs. 



MARCHI'S methods for the tendons of the motores bulbi oculi 

 (Archivio per le Scienze Mediche, v, No. 15). The enucleated eyes, 

 together with their muscles, were put for not less than three days 

 into 2 per cent, bichromate of potash. The muscles and tendons 

 were then carefully dissected out, stained with gold chloride and 

 osmic acid (G-OLGi's method), and by the method of MANFREDI, 

 368. Mount in glycerin. The methods only succeed completely 

 during fine, sunny weather. 



RUFFINI (Atti R. Ace. Lined Roma Rend. [5], i, 1892, p. 442) 

 recommends the method of Fischer. 



CIACCIO (Mem. R. Ace. Sci. Bologna [4], t. x, 1890, p. 301) puts 

 tendons of Amphibia into 0-1 per cent, hydrochloric acid or 0-2 

 per cent, acetic acid until transparent ; then for five minutes into 

 a mixture of 0-1 per cent, gold chloride and 0-1 per cent, potassium 

 chloride ; then back into the acetic acid, for a day in the dark, and 

 for two or three hours more in the sunlight. When they have 

 become somewhat violet they are put for a day into 0-1 per cent, 

 osmic acid, and finally mounted in glycerin acidulated with 0-5 

 per cent, of acetic or formic acid. 



DOGIEL (Arch. mik. Anat., Ixvii, 1906, p. 638) stretches tendons 

 of eye-muscles on cardboard with hedgehog spines, puts for four 



