348 MUSCLE AND TENDON (NERVE-ENDINGS). 



or five days into nitrate of silver of 1 to 2 per cent., reduces for a 

 day in pyrogallic acid with formol, and imbeds in celloidin. 



Smooth Muscle. 



737.- Tests for Smooth Muscle. Picro-saurefuchsin, 299, stains 

 muscle yellow, connective tissue red. 



Picro-nigrosin, 325, stains muscle yellowish, connective tissue 

 blue. 



UNNA (Encycl. mik. Technik., article " Kollagen ") stains for 

 twenty-four hours in orcein 1 part, Wasserblau 0-25, alcohol 60, 

 glycerin 10, water 30, which gives muscle in a mixed tone, collagen 

 blue, elastin reddish. See also a complicated process with methylen 

 blue in Monctssch. prakt. DermatoL, xix, 1894, p. 533, and another 

 with orcein, hsematein, saurefuchsin and picric acid. 



RETTERER (C. R. Soc. BioL, 1887, p. 645) fixes in 10 volumes of 

 alcohol with one of formic acid, washes well and stains in alum 

 carmine. Muscle red, connective tissue unstained. 



738. General Structure. WERNER (Hist. d. glatten Musculatur, 

 Dorpat, 1894, p. 22) fixes stretched intestine or bladder in Flemming, 

 washes well and stains in Heidenhain's chrome haematoxylin, 265. 

 For demonstrating intercellular spaces, fresh intestine is put for 

 twenty-four hours into oil, at 37 C., then for twelve hours into 

 Flemming, and for four to six into chromo-acetic acid. 



739. Isolation of Fibres. GAGE'S methods, see 518, 528, and 

 536. 



MOBIUS, muscle of Cardiwn, see 527. 



BALLOWITZ, muscle of Cephalopoda, see Arch. mik. Anat., xxxix, 



1892, p. 291. 



SCHULTZ (Arch. Anat. Phys., Phys. Abth., 1895-6, p. 521) puts 

 muscle of Vertebrates for twenty-four hours into 10 per cent, nitric 

 acid, rinses with water, and brings pieces for six to eight days (in 

 the dark at first) into a mixture of equal parts of -f r> per cent, osmic 

 acid and J per cent, acetic acid, teases and mounts in glycerin. 



For smooth muscle of Vermes, see APATHY, Zeit. f. wiss. Mik., x, 



1893, pp. 36, 319, and 530, ante. 



740. Iris. DOGIEL (Arch. mik. Anat., 1886, p. 403) puts the 

 anterior half of an enucleated eye for some days into a mixture of 

 two parts one-third alcohol and one part 0-5 per cent, acetic acid. 

 The iris can then be isolated, and split from the edge into an interior 

 and posterior plate, and these stained according to the usual methods. 



