CHAPTER XXVI I L 349 



See also KOGANEI, Arch. mik. Anat., 1885, p. 1 ; CANFIELD, ibid., 

 1886, p. 121 ; and DOSTOIEWSKY, ibid., p. 91. 



741. Bladder of Frog, Innervation of (WOLFF, Arch./, mik. Anat., 

 1881, p. 362). A frog is killed and a solution of gold chloride of 

 1 : 20,000 injected into the bladder through the anus. (If the 

 injection flows out on removal of the syringe, tie the frog's thighs 

 together.) Now open the frog, ligature the intestine above the 

 bladder, and cut away the abdomen so as to have in one piece 

 bladder, rectum and hind legs. Put this into gold solution of 

 1 : 2000 for four hours ; the bladder is then excised, slit open, and 

 pinned (with hedgehog spines) on to a cork (outside downwards). 

 Place it under running water until all the epithelium is washed 

 away. Use a camel's-hair brush if necessary. Put for twenty -four 

 hours into gold solution of 1 : 6000. Wash in pure water, and put 

 away in the dark " for some time " in acidulated water, and finally 

 reduce in fresh water in daylight. 



RANVIER (Traite, p. 854) recommends his two gold processes, 

 the liquids being injected as above. 



GRUNSTEIN (Arch. mik. Anat., 1899, p. 1) injects, 1 per cent, 

 methylen blue in normal salt solution through the vena abdominalis, 

 and after twenty to thirty minutes excises the bladder and exposes 

 to the air. Fix the stain with picrate of ammonia and mount in 

 glycerin with the same ( 343). 



