376 CONNECTIVE TISSUES. 



in iron-hsematoxylin ( 679), and the Golgi apparatus in these cells is 

 .well shown by the employment of Golgi's method, Cajal's method, 

 or of Da Fano's modification thereof ( 844, 849), though a negative 

 image of this cell-element is clearly shown when the tissues are 

 fixed in Sansom's modification of Carnoy's mixture. 



777. Bone, Decalcified (FLEMMING, Zeit. wiss. Mik., 1886, p. 47). 

 Sections of decalcified bone are soaked in water, dehydrated with 

 alcohol under pressure, dried under pressure and mounted in hard 

 balsam melted on the slide. They show the lacunar system injected 

 with air as in non-decalcified sections. 



778. Stains for Cartilage and Decalcified Bone. See hereon 

 SCHAFFER in Zeit. wiss. MiL, v, 1888, p. 1 ; and .Encyd. mik. 

 Technik., art. " Knochen." 



KOLLIKER (Zeit. wiss. Zool, xliv, 1886, p. 662) treats sections of 

 decalcified bone with concentrated acetic acid until they become 

 transparent, and then puts for one quarter to one minute into a 

 concentrated solution of indigo-carmine, washes and mounts in 

 ' glycerin or balsam. The fil^es_o Sharpey appear red, the remaining 

 bone substance blue. 



SCHAFFER (Zeit. wiss. Mik., v, 1888. p. 17) employed at one 

 time a safranin method modified from BOUMA (Centralb. med. Wiss., 

 1883, p. 866), for which see previous editions. He now (Encyd. mik. 

 Tech., 1910, i, p. 762) stains sections for twenty-four hours in a 

 bath of 20 c.c. of water with 1 drop of 1 per cent, solution of safranin 

 (or thionin) and (apparently) mounts in balsam. The safranin stain 

 will keep if the material is cartilage which has been fixed in picro- 

 sublimate ; otherwise it must be fixed with ammonium molybdate 

 of 5 per cent, before dehydrating. 



SCHMORL (Centralb. allg. Path., x, 1899, p. 745) stains in a.mixture 

 of 2 c.c. concentrated solution of thionin in alcohol of 50 per cent, 

 and 10 c.c. of water for ten minutes, rinses and puts into saturated 

 aqueous picric acid for thirty to sixty seconds. Kinse and pass 

 through graded alcohols into origanum oil or carbol-xylol and 

 balsam. Matrix yellow, cells red, fat-cells violet. He also describes 

 a more complicated method with thionin and phosphotungstic or 

 phosphomolybdic acid. 



MOLL (Centralb. PJiysiol., xiii, 1899, p. 225) stains embryonic 

 cartilage for six to twenty-four hours in orcein 0-5 gr., alcohol 40, 

 water 20, hydrochloric acid 20 drops, and mounts iiTbalsam. Matrix 

 blue, nuclei red. 



KALLIUS (Anat. Hefte, xxx, 1905, p. 9) stains first with borax 

 carmine or alum-carmine, then (sections) for ten minutes in satu- 



