CHAPTER XXX. 383 



hsemoglobigenous granules in five to ten minutes. This is also 

 recommended by EHRLICH and LAZARUS. See 309. 



Similarly also Ross (Trans. Path. Soc., 1907, p. 117), using 

 polychrome methylen blue. 



LEVADITI (Journ. Phys. path. Gen., Paris, 1901, p. 425) allows 

 solution of Brillantkresylblau in alcohol to dry on a slide, puts a 

 drop of blood on the dried layer, and covers. Similarly CESARIS- 

 DEMEL (Arch. path. Anat., 1909, p. 92), with a mixture of this dye 

 and Sudan III ; and NAKANISHI (Centralb. Bakt., 1901, p. 98), with 

 methylen blue BB. 



Fixed films may be treated with the usual tissue stains, eosin 

 being an important one, as it stains rose-red all parts of blood-cells 

 that contain haemoglobin. EHRLICH'S acid hsematoxylin, with 

 0-5 gr. of eosin dissolved in it, is a good general stain. Or, stain 

 with hsemalum, and then with eosin (0-5 per cent, in alcohol or 

 water). 



EHRLICH'S triacid ( 296) gives good general views, and demon- 

 strates neutrophilous granules. His mixture for eosinophilous 

 cells has been given (311). 



PAPPENHEIM'S panoptic triacid (on sale by Griibler) is Ehrlich's 

 triacid with methylen blue in place of the methyl green. 



CHENZINSKI'S mixture, which is good, has been given ( 313). 

 Stain for six to twenty-four hours in a stove. This gives rise to 

 precipitates. To avoid them (WILLEBRAND, Deutsch. med. 

 Wochenschr., 1901, p. 57) you may make a mixture of equal parts 

 of 0-5 per cent, solution of eosin in 70 per cent, alcohol and saturated 

 solution of methylen blue in water, and add acetic acid of 1 per 

 cent, drop by drop till the mixture begins to turn red, and filter 

 before use. Or (MICHAELIS, ibid., 1899, No. 30) make (a) a mixture 

 of 20 parts 1 per cent, aqueous methylen blue with 20 of absolute 

 alcohol, and (b) a mixture of 12 parts 1 per cent, aqueous eosin with 

 28 of acetone, and for staining mix equal parts of these and stain 

 for half a minute to ten minutes. 



JENNER (Lancet, 1899, No. 6, p. 370) mixes equal parts of 1-2 to 

 1-25 per cent, water-soluble eosin (Griibler's) and 1 per cent, methylen 

 blue, filters after twenty-four hours, washes the precipitate on the 

 filter, dries it, and dissolves it in 200 parts of absolute methyl alcohol 

 (the solution can be had ready made from Griibler and Hollborn). 

 (Or, simply mix 125 c.c. of 0-5 per cent, solution of the eosin in 

 methyl alcohol with 100 c.c. of 0-5 per cent, solution of methylen 

 blue.) Cover-glass films are floated on to this, in which they are 

 fixed and stained in three minutes. Wash off the stain with a little 



