386 BLOOD AND GLANDS. 



first stained for five to ten minutes in solution of 1 part methy- 

 lenblau med. puriss. Hoechst, 0-5 part of lithium carbonate and 

 100 of water, kept for at least three weeks and diluted with 10 

 volumes of water. Rinse with water, dry with blotting paper^ 

 flood with JENNER'S solution diluted with 2 or 3 volumes of water, 

 and stain for five to ten minutes. Wash, dry with blotting paper, 

 and mount. See also SCOTT, Folia Haem., xii, 1911. 



785. PAPPENHEIM (Anat. Anz., xlii, 1912, p. 525) recommends the 

 following for sections of Jicemopoietic tissues, and also of kidney, liver, 

 hypophysis, suprarenals, lung, intestinal epithelium and central nervous 

 system. Fix in Orth's Formol-Muller, stain sections for twenty minutes 

 in a stove in " aqueous diluted alcoholic " solution of MAY-GRUNWALD 

 or JENNER diluted with 8 volumes of water ; after-stain for forty 

 minutes in the stove in " aqueous GIEMSA solution (15 c.c. of water with 

 10 drops of glacial acetic acid) " ; differentiate in 100 c.c. of water with 

 5 to 6 drops of acetic acid ; wash, dry between blotting-paper ; dehydrate 

 in mixture of equal parts of- acetone and absolute alcohol, and mount in 

 neutral balsam. The result is not a Eomanowsky stain, but a pale 

 methylen-blue-eosin stain. 



See also WRIGHT, Pub. Massachusetts Gen. Hosp., iii, 1910, p. 1, or 

 Journ. E. Micr. Soc., 1910, p. 783. 



For the special technique of eosinophilous cells see MARTINOTTI in 

 Zeit. wiss. Mikr., xxvi, 1909, p. 4 (alphabetical bibliography of eight 

 pages). 



786. Demonstration of Blood-plates of Bizzozero (KEMP, Studies 

 from the Biol. Lab. Johns Hopkins Univ., May, 1886, iii, No. 6 ; 

 Nature, 1886, p. 132). A somewhat large drop of blood is placed 

 on a slide, and quickly washed with a small stream of normal salt 

 solution. The blood-plates are not washed away, because they 

 have the property of adhering to glass. They may be stained with 

 solution of 0-02 per cent, of methyl violet or 1 : 3,000 of gentian 

 violet, in salt solution. To make permanent preparations of them, 

 they should first be fixed, by putting a drop of osmic acid solution 

 on the finger before pricking it. 



They may also be stained in films, especially by the Romanowsky 

 method. According to PAPPENHEIM (Farbchemie, p. 107) Wasser- 

 blau is almost specific for them. 



WRIGHT (Journ. Morph., xxi, 1910, p. 274) studies them in tissues, 

 after fixation with formol or sublimate (not Zenker) by staining 

 with a modified Giemsa stain, and bringing through acetone and oil 

 of turpentine into turpentine colophonium. Details loc. cit. or 

 Journ. Roy. Mic. Soc., 1910, p. 783. 



Bee also DEKHUYZEN, Anat. Anz.. xix, 1901, p. 533; KOPSCH, 



