392 BLOOD AND GLANDS. 



cent, acetic acid, or in a similar solution of neutral red (two to three 

 days, washing out with alcohol). 



MAYER (Mitt. Zool. Stat. Neapel.,xu, 1896, p. 303, or last edition) gives 

 the following two formulae for mixtures that stain exclusively mucus. 



793. MAYER'S Mucicarmine (op. cit., last ). One gramme of carmine, 

 and 0-5 grm. of aluminium chloride with 2 c.c. of distilled water heated 

 over a small flame for two minutes, and made up to 100 c.c. with 50 per 

 cent, alcohol. This gives a stock solution, which is as a rule to be 

 diluted for use tenfold with distilled or tap water. 



MAYER'S Muchaematein (ibid.). Hsematein 0-2 grm., aluminium 

 chloride 0-1 grm., glycerin 40 c.c., water 60 c.c. An alcoholic solution 

 may be made by dissolving in 100 c.c. of 70 per cent, alcohol, with or 

 without the addition of 2 drops of nitric acid. 



794. Mucicarminic Acid (KAWITZ, Anat. Am., xv, 1899, p. 439). 

 One gramme of carminic acid, 2 of aluminium chloride, and 100 c.c. of 

 50 per cent, alcohol. 



795. Goblet Cells. So far as these contain mucin they give the 

 reactions above described. See PANETH, Arch. mik. Anat., xxxi, 

 1888, pp. 113 et seq. ; LIST, ibid., xxvii, 1886, p. 481 ; and GUYEISSE, 

 C. R. Soc. Biol, 1907, p. 1212. 



For intestinal epithelium, especially the cells of PANETH, see also 

 MARTIN, Unters. ueb. Oberfldchen u. Drusenepithel, Leipzig, 1910 ; 

 and KULL, Arch. mik. Anat., Ixxvii, 1911, p. 541 (sections stained 

 with alum hsematoxylin, treated for twenty to thirty seconds with 

 tincture of iodine, stained a few minutes with Victoria blue, then 

 with eosin). 



796. Salivary Glands. SOLGER (Unters. z. Naturlehre d. Menschen 

 xv, 5 and 6, pp. 215; Festschr. f. Gegenbaur, ii, 1896, p. 21P 

 demonstrates the granules in serous cells and half -moons of the 

 submaxillary gland by hardening in a 10 per cent, solution of formol, 

 and then making sections and staining with hsematoxylin of Delafield 

 or of Ehrlich, the granules taking the stain. 



KRAUSE (Arch. mik. Anat., xlv, 1895, p. 94) stains sections either 

 with Heidenhain's iron hsematoxylin or with Ehrlich-Biondi mixture 

 or thionin. See also KRAUSE, ibid., xlix, 1897, p. 709 ; and MULLER, 

 Zeit. wiss. Zool, 1898, p. 640. 



797. Gastric Glands. KOLSTER (Zeit. wiss. Mik., xii, 1895, 

 p. 314) differentiates the two kinds of cells in stomach glands by 

 over-staining with hsematoxylin, washing out with alcohol containing 

 1 per cent, of HC1, blueing with alcohol containing 1 per cent, of 

 ammonia, and, after washing, staining for one to five minutes in a 



