CHAPTER XXX. 393 



weak solution of Saurefuchsin. Peptic cells blue, parietal cells red. 

 Osmic material cannot be employed. 



CADE (Arch. Anat. Micr., iv, 1901, p. 4) stains material fixed 

 with Bourn's picroformol in Victoria blue of 1 per cent. 



B. and L. MONTI (Rich. Lab. Anat. Roma, ix, 1902) demonstrate 

 ducts and canaliculi of delomorphous cells by Golgi's bichromate 

 and silver impregnation, especially with rejuvenated material (see 

 SACERDOTTI), leaving it for five or six days in half -saturated sulphate 

 of copper, then for twenty-four hours in the osmic-bichromate 

 mixture. You can embed in paraffin (rapidly). 



798. Intestine. BENSLEY (Amer. Journ. Anat., v, 1906, p. 323) 

 stains sections of glands of Lieberkiihn in a mixture of equal parts 

 of saturated solutions of orange G and Saurerubin, and then with 

 toluidin blue, and mounts in balsam. 



799. Liver. BRAUS (Denkschr. Med. Nat. Ges. Jena, v, 1896, 

 p. 307) demonstrates the bile capillaries by the rapid method of 

 GOLGI, hardening in a mixture of 1 part formol with 3 parts liquid 

 of Miiller or J per cent., chromic acid. 



EPPINGER (Beitr. path. Anat., xxxi, 1902, p. 230) studies them 

 by means of a complicated modification of WEIGERT'S neuroglia 

 stain, and CIECHANOWSKI (Anat. Anz., xxi., 1902, p. 426) by means 

 of WEIGERT'S myelin stain (the 1885 method). 



OPPEL (Anat. Anz., v, 1890, p. 144 ; vi, 1891, p. 168) puts pieces 

 of liver or spleen (alcohol material) for twenty-four hours into a 

 solution of neutral chromate of potash (J to 10 per cent.), then for 

 twenty-four hours into a f per cent, solution of silver nitrate, washes, 

 dehydrates and cuts without embedding. The lattice fibres are only 

 stained near the surface, so that tangential sections must be made. 



Similarly BERKLEY, ibid., 1893, p. 772, fixing in picric acid, then 

 in an osmium bichromate mixture, and then silvering. 



See also RANVIER, Journ., de Microgr., ix, x, 1885-6 ; IGACUSCHI, in 

 Arch. path. Anat., xcvii, p. 142, or Zeit. wiss. Mile., 1885, p. 243 (gold 

 process for study of fibrous networks) ; KUPFPER, Sttzb. Ges. f. Morph., 

 etc., Miinchen, Juli, 1889, or Zeit. wiss. Mik., vi, 1889, p. 506 ; KRAUSE 

 (Arch. mik. Anat., xlii, 1893, p. 57) ; and TIMOFEJEW, Anat. Anz., xxxv, 

 1909, p. 296 (sections of frozen tissue stained with methylen blue). 



800. Spleen. For lattice fibres, see OPPEL, last . 



KULTSCHITZKY (Arch. mik. Anat., xlvi, 1895, p. 675) studies 

 the musculature in sections (of material from liquid of Miiller) 

 stained for a day or more in a solution of lakmoid in ether and 

 mounted in balsam. 



