394 BLOOD AND GLANDS. 



For elastic fibres he puts sections for half an hour or a day into 

 a mixture of 800 parts 96 per cent, alcohol, 40 parts 1 per cent, 

 solution of carbonate of potash, 2 parts Magdala red, and 1 part 

 methylen blue. 



For the blood vessels he puts sections of Miiller material for a 

 few minutes into a solution of 1 or 2 parts of Saurerubin in 400 parts 

 of 3 per cent, acetic acid, washes out in 2 per cent, acetic acid, and 

 after-stains in a similar solution of helianthin or Wasserblau until 

 the red only remains in the erythrocytes. 



See also WHITING (Trans. Roy. Soc., Edinburgh, xxxviii, 1896, p. 311) ; 

 SCHUMACHER (Arch. mik. Anat., Iv, 1899, p. 151) ; WEIDENREICH (ibid., 

 Iviii, 1901, p. 251). 



801. Lymphatic Glands. For lattice-fibres especially, see EOESSLE 

 and YOSHIDA, Beitr. path. Anat., xlv, 1909, p. 110, or Zeit. wiss. 

 Mik., xxvi, 1909, p. 295. Sections stained with hsematoxylin and 

 eosin, or Weigert's iron haematoxylin, or Bielschowsky's neurofibril 

 stain as applied by MARESCH, loc. cit., 752. The sections should 

 not remain for more than fifteen to thirty minutes in the oxide bath. 



See also for the thymus some very complicated methods of SAL- 

 KIND, Anat. Anz., xli, 1912, Nos. 6 and 7. 



802. Kidney. SAUER (Arch. mik. Anat., xlvi, 1895, p. 110) finds 

 that for the renal epithelium the best fixative is Carnoy's acetic 

 alcohol with chloroform (three to five hours, washing out with 

 absolute alcohol). A mixture of 9 parts alcohol with 1 of nitric 

 acid is also good, as is liquid of Perenyi. He stains with iron 

 haematoxylin, and after-stains in a very weak solution of Saurerubin 

 in 90 per cent, alcohol, which stains the ciliary plateau. He 

 macerates with iodised serum or one-third alcohol, staining after- 

 wards with dahlia. 



ARNOLD (Anat. Anz., xxi, 1902, p. 417) employs intra vitam 

 staining methods for the study of the granules of the epithelium 

 cells. Sections of fresh kidney are cut with a Valentin's knife, and 

 brought into a very dilute solution of neutral red, or methylen blue, 

 in which the granules stain in a few minutes or hours. Or saturated 

 solutions of the dyes, or of indigo carmine, may be injected sub- 

 cutaneously during life, at intervals of fifteen to twenty minutes, 

 and after two to five injections the organ may be excised and sections 

 made and examined (see 208 and 342 to 344). 



803. Thyroid. BENSLEY (Amer. Journ. Anat., xxix, 1916) uses 

 brazilin and water blue. Fix gland in Zenker-formol. Section in 

 paraffin and fix sections to slide with water alone, or very little 



