408 NERVOUS SYSTEM GENERAL METHODS. 



817. Nigrosin and Anilin-Blue-Black. Nigrosin has given useful 

 results in some hands. Aniliii-blue-black has been much recommended 

 by SANKEY (Lancet, 2, 1875, p. 82) ; BEYAN LEWIS (Human Brain, 

 p. 125, and Quart. Journ. Micr. Sc., 1876, pp. 73-75) ; VEJAS (Arch. f. 

 Psych, xvi, 1885, p. 200) ; MARTINOTTI (ii, 1885, p. 478) ; JELGERSMA 

 (Ztschr. wiss. Mikr., 1886, p. 39) : SCHMAUS (Munch, med. Wochenschr., 

 1891. p. 147), and others. And see also previous editions. 



818. Picronigrosin. MAKTINOTTI (loc. cit., 1885, p. 478) stains 

 for two or three hours or days in a saturated solution of nigrosin in 

 saturated solution of picric acid in alcohol, and washes out in a 

 mixture of 1 part of formic acid with 2 parts of alcohol. 



819. KAISER (Ztschr. wiss. Mikr., vi, 1889, p. 471) stains sections of 

 spinal cord for a few hours in a solution of 1 part of naphthylamin brown, 

 200 of water, and 100 of alcohol, washes with alcohol, clears with 

 origanum oil and mounts. 



820. Alizarine. SCHE OTTER (Neurol. Centrbl, xxi, 1902, p. 338) 

 stains sections for twenty-four hours in a 1 to 2 per cent, solution 

 of sulphalizarinate of soda, differentiates for half to one minute in 

 tap-water, dehydrates, and mounts. This is a general stain, but 

 demonstrates Nissl bodies and other internal details. 



821 MALLORY'S Phosphomolybdic Acid Hsematoxylin and KODIS' 

 modification, see 271. For AUERBACH'S modification, see Neurol. 

 Centrbl., xvi, 1897, p. 439. 



822. Hsematoxylin and Acid Fuchsin. FINOTTI (Virchow's Arch., 

 cxliii, 1896, p. 133) stains in hsematoxylin, counterstains for three 

 minutes with 0\5 to 1 per cent, solution of acid fiichsin, and differen- 

 tiates in 75 per cent, alcohol containing a very little caustic potash. 



VAN GIBSON'S hsematoxylin and picro-fuchsin ( 398) may give useful 

 general views of nerve-cells, axis-cylinders, and ncuroglia. 



823. Other General Stains. ALT (Munch, med. Wochenschr., 1892, 

 No. 4) stains for a couple of hours in a solution of Congo red in absolute 

 alcohol, and washes out with pure alcohol. This is useful for peripheral 

 axis-cylinders and other elements. 



SCARPATETTI (Neurol Centrbl, xvi, 1897, p. 211) stains sections of 

 formol material for five minutes in 1 per cent, hsematoxylin, treats for 

 five minutes with concentrated solution of neutral copper acetate, 

 differentiates with Weigert's borax-ferricyanide, treats with con- 

 centrated solution of lithium carbonate, washes and mounts. Myelin 

 is not stained. 



ROTIIIG (Folia Neurobiol, ii, 1909, p. 385) fixes and stains for about 

 four weeks in saturated solution of methylenazur I., in 10 per cent, 

 formol, puts for ten to fifteen minutes into acetone, then for twelve 

 hours into chloroform, and imbeds in paraffin. He also has a process 

 with trichloracetate of lead and methylenazur. 



