CHAPTER XXXII. 413 



celloidin if the sections are, whenever possible, freed from the 

 celloidin before staining them, and if they are re-stained a second and 

 .a third time after having been each time completely differentiated. 

 See DA FANO, Proc. Physiol. Soc., Journ. Physiol., liv, 1920-1. 



All preparations stained by Nissl's method keep badly, but they 

 keep a little better and may last almost unchanged even for 

 years : (1) if the anilin-alcohol or alcohol used for the differentiation 

 is properly washed away with pure benzol ; (2) if the xylol-colo- 

 phonium for the mount is prepared with pure xylol, and of the 

 thickness needed ; (3) if sections of material embedded in celloidin 

 are submitted to the above-mentioned succession of staining and 

 differentiation ; (4) if preparations are carefully protected from 

 light. 



828. Modifications of Nissl's Method. REHM (Munch, med. 

 Wochenschr., xxxix, 1892, p. 217) floats sections for half a minute 

 to a minute on a hot 0-1 per cent, solution of methylene blue, 

 differentiates them in 96 per cent, alcohol and clears them with 

 origanum oil. 



LENHOSSEK (Fein. Ban. d. Nervens., 1895) stains sections of 

 formol material in a concentrated aqueous solution of thionine, rinses 

 them with water and mounts them like Nissl. 



LUXENBURG (N enrol. Centrbl, xviii, 1899, p. 629) stains paraffin 

 serial sections either with Nissl's methylene blue or with thionine as 

 Lenhossek. 



JULIUSBURGER (Neurol. Centrbl., xvi, 1897, p. 259) stains sections 

 of material fixed in Orth's fluid and embedded in celloidin, either 

 with Nissl's methylene blue or with warmed neutral red. 



ROSIN (Deutsche med. Wochenschr., xxiv, 1898, p. 615) treats 

 sections of formol material similarly. 



LENHOSSEK (Neurol. Centrbl., xvii, 1898, p. 577) stains paraffin or 

 celloidin sections of spinal ganglia fixed in Carney's fluid with a 

 concentrated watery solution of toluidine blue overnight, rinses with 

 water, differentiates quickly with alcohol and clears with xylol or 

 carbol-xylol. 



POLUMORDWINOW (Ztschr. wiss. Mikr., xvi, 1899, p. 371), uses 

 1 part of 1 per cent, solution of toluidine blue to 119 of distilled 

 water and 1 of sodium carbonate. 



VAN GEHUCHTEN and NELIS (La Cellule, xiv, 1898, p. 374) recom- 

 mend fixing spinal ganglia in Gilson's mixture. 



VAN GEHUCHTEN (see 1913 ed.) uses paraffin sections mounted 

 on slides by the water method and stains them for five to six 



