414 NERVOUS SYSTEM SPECIAL METHODS. 



hours in Nissl's methylene blue solution in the incubator at 35 

 to 40 C. 



GOTHARD (C. R. Soc. BioL, v, 1898, p. 330) stains celloidin sections 

 for twenty-four hours in Unna's polychrome methylene blue, and 

 differentiates them with a mixture of 5 parts of creosote, 4 of cajeput 

 oil, 5 to 8 of xylol, and 16 of absolute alcohol. The mixture is 

 removed with absolute alcohol and sections mounted in xylol dammar 

 after clearing with cajeput oil. 



LUITHLEN and SORGO (N enrol. Centrbl., xvii, 1898, p. 640) diffe- 

 rentiate in Unna's glycerin-ether mixture, remove this with absolute 

 alcohol, and clear with origanum oil. 



Similarly LENNHOFF (ibid., 1910, p. 20) ; or, polychrome methylene 

 blue two minutes, distilled water quickly, carbol-pyronin-methyl 

 green twenty minutes ; distilled water quickly, absolute alcohol, 

 oil, balsam. 



LORD (Journ. Ment. Sc., xliv, 1898, p. 693) makes sections from 

 frozen fresh tissues, treats them for a few seconds with a mixture of 

 equal parts of 6 per cent, formaldehyde and saturated solution of 

 picric acid, then rinses them with distilled water and stains them in 



5 per cent, solution of methylene blue B pat. 



829. BIELSCHOWSKY and PLIEN'S Cresyl Violet Method (Neural. 

 Centrbl., xix, 1900, p. 1141). Celloidin or paraffin sections of material 

 fixed either in alcohol or formalin, or sections made by the freezing 

 method from formalin material, are stained for twenty-four hours 

 in a very diluted solution of cresyl violet R.K., prepared by adding 



6 to 8 drops of a concentrated aqueous solution to every 50 c.c. of 

 distilled water. After a quick wash in distilled water sections are 

 brought through the ascending series of alcohols, cajeput oil, and 

 xylol, into balsam. The preparations are said to keep better than 

 those stained with thionine or toluidine blue. 



830. Picrocarmine has been successfully used by MESSNER (Journ. 

 Psychol. Neurol., xviii, 1912, p. 204, and xx, 1913, p. 256). Sections 

 of alcohol material, embedded in celloidin or not, are washed in water 

 and then stained for five minutes in a warmed diluted solution of 

 Ranvier's picrocarmine. After a quick wash, they are differen- 

 tiated in 3 per cent, hydrochloric acid, dehydrated and mounted 

 as usual. In the case of the spinal cord, medulla oblongata and 

 pons the method succeeds also if material was fixed in formalin. 



831. Other Methods for Tigroid Substance and Basophil Granules 

 in General. See GOLDSCHEIDER u. FLATAU, Norm. u. path. Anat. d. 

 Nervenz, Berlin, 1898, or Ztschr. wiss. Mikr., xvi, 1899, p. 102, and 



