448 MYELIN STAINS. 



washed, and stained for fifteen to twenty minutes in a lithium 

 carbonate hsematoxylin similar to Weigert's, warmed to 40 C., 

 allowed to cool, and differentiated for one to three minutes in 

 Weigert's ferricyanide liquid, which may be diluted if desired with 

 one third of water. 



HILL (Brain, xix, 1896, p. 1 ; Phil Trans., 184, B, 1894, 

 p. 399) stains well-washed Miiller material in bulk in alum carmine, 

 cuts and mordants sections for twenty-four hours in half-saturated 

 solution of copper acetate, stains and differentiates as Weigert, 

 taking the differentiating fluid only half as strong. 



863. BENDA'S Rapid Method (Berlin klin. Wochenschr., xl, 1903, 

 p. 748). Sections of formal material by the freezing process (alcohol being 

 avoided) are stained (without any mordanting) for twenty-four hours in 

 Boehmer's hsematoxylin, differentiated with Weigert's ferricyanide, and 

 mounted in balsam. Only recommended for peripheral nerves, or for 

 preliminary examination of the central nervous system. 



Similarly, NAGEOTTE, C.R. Soc. Biol., ii, 1908, p. 408, staining with 

 hsemalum. 



Similarly the Enzycl. mile. Technik., 1910, ii, p. 239, with fresh material 

 cut by the freezing process, and the sections mounted in laevulose (as 

 alcohol somewhat extracts the stain). 



864. STREETER (Arch. mik. Anat., Ixii, 1903, p. 734) stains small 

 nerve-centres in bulk (after mordanting in Weigert's bichromate and 

 fluoride mixture, 845) with Weigert's haematoxylin (four to six 

 days), washes for a couple of days in 70 per cent, alcohol, makes 

 paraffin sections, and differentiates them by the method of Weigert 

 or Pal. 



865. BESTA'S Ammonio-Chloride of Tin Methods (Riv. Sperim. 

 Freniatr., xxxi, 1905, p. 569). Pieces of peripheral nerves are fixed 

 for one to three days in 100 c.c. of water with 25 of formol, and 4 grms. 

 of Merk's ammonio-chloride of tin, and then dehydrated and em- 

 bedded as usual. The sections may be stained in different ways : 

 (a) For twenty-four hours in Mallory's phosphomolybdic-carbolic- 

 acid hsematoxylin with subsequent differentiation in Lugol's solu- 

 tion ; (b) for thirty to sixty minutes in a very diluted solution of 

 Delafield's hsematoxylin and then for a minute in Held's acetic 

 solution of erythrosin ; (c) for five to ten minutes in erythrosin, 

 and then for two hours in a mixture of equal parts of 1 per cent, 

 hsematoxylin and 4 per cent, ammonium molybdate with 3 drops of 

 acetic acid to every 50 c.c. of the mixture. 



866. Gallein. ARONSON (Centrbl. med. Wise., xxviii, 1890, p. 577) stains 

 sections of material, hardened in liquid of Erlicki or Miiller and mor- 



