476 AXIS-CYLINDER AND DENDRITE STAINS. 



for ten minutes in water, stained for twenty-four hours in an incu- 

 bator in Kultschitzky's haematoxylin, and differentiated in 80 per 

 cent, alcohol acidified with 0-5 per cent, of hydrochloric acid until 

 slightly blue-red. The acid is then removed by washing with pure 

 alcohol, and the sections dehydrated, cleared with origanum oil, 

 and mounted in balsam. Axis-cylinders, nerve-cells and glia cells 

 are stained, the myelin being coloured only when the differentiation 

 in the acid alcohol has been insufficient. 



901. AZOULAY'S Ammonium Vanadate Process (Bull. Soc. Anat., 

 Paris, Ixix, 1894, 5th S., p. 924). Wash in water thin sections of 

 material fixed in a bichromate solution and embedded in celloidin. 

 Lift a section on a slide and pour on it a few drops of -5 per cent, 

 ammonium vanadate, wait a moment, pour off the stain, wash with 

 a little distilled water and pour on the section a few drops of 2 -5 per 

 cent, tannin. After a few minutes pour off the tannin solution, 

 wash, and start all over again, and so on until axis-cylinder and 

 nerve-cells are stained dark green. Wash quickly, dehydrate and 

 mount. These preparations photograph well. 



902. FAJERSTAJN'S Haematoxylin (Poln. Arch. Biol. Med. Wiss., 

 i, 1901, p. 189). Make sections, by means of the freezing microtome, 

 of material fixed for two to seven days in 5 to 10 per cent, formalin. 

 Transfer them into 0-25 to 0-5 per cent, chromic acid, and after 

 twenty-four hours wash them well, and put them to stain for another 

 twenty-four hours in 1 per cent, aqueous solution of hsematoxylin 

 Differentiate by Pal's method. 



903. NABIAS' Method (C. R. Soc. Biol, Ivi, 1904, p. 426). Sections 

 of material fixed in alcohol-corrosive sublimate or any other fixing 

 agent easily allowing the penetration of iodine are treated until 

 yellow with Lugol's solution (Gram's formula). They are then 

 quickly washed, and treated for a few minutes with 1 per cent, 

 gold chloride, quickly washed once more, and reduced in 1 per cent, 

 watery solution of anilin oil or resorcin. Dehydrate and mount in 

 balsam. 



904. LENNHOFF'S Processes (Neurol. Centrbl, xxix, 1910, p. 20). 

 (1) Polychrome-methylene blue and potassium sulphocyanide method 

 for axis-cylinders : Fixation not stated. Stain sections in polychrome 

 methylene blue for two to five minutes, wash them in distilled water and 

 transfer them for half to twenty-four hours into potassium sulpho- 

 cyanide (strength not stated). Wash, dehydrate, clear, and mount in 

 balsam. (2) Polychrome-methylene blue and potassium fcrricyanide 

 method for axis -cylinders and nerve cells. Sections of material fixed 

 in alcohol are treated as above, using potassium ferricyanide instead of 



