CHAPTER XXXV. 483 



and 45 0., so that pure paraffin, melting at 58 C., is used only for 

 the embedding proper. 



The sections, stuck to slides, are mordanted for twenty-four hours 

 in 4 per cent, iron alum or in 50 per cent. Liquor ferri sulfurici 

 oxydati P.G-., thoroughly washed, put for. two hours into an amber- 

 yellow aqueous solution of sodium sulfalizarinate as directed in 

 683, rinsed with tap water, and put to stain in 0*1 per cent, toluidine 

 blue either for fifteen minutes by warming until vapour arises, or 

 for twenty-four hours at room temperature. After rinsing in 



1 per cent, acetic acid or in a very dilute solution of picric acid, the 

 sections are dried with filter paper, passed through absolute alcohol, 

 and differentiated for about ten minutes with creosote. They are 

 then dried once more with filter paper, washed with xylol and 

 mounted in balsam. 



Besides this, Benda recommends hardening and making paraffin 

 sections as above, then staining by Weigert's method ( 910), but 

 without passing the sections through the saturated solution of 

 " chromogen," and using instead of Weigert's methyl violet solution 

 a freshly prepared mixture of 1 volume of saturated solution of 

 crystal violet, 1 volume of 1 per cent, acid alcohol, and 2 volumes of 

 aniline water. 



Benda also uses Heidenhain's iron haematoxylin to stain paraffin 

 sections of pieces treated as described, differentiating either with 



2 per cent, iron alum or with Weigert's borax-ferricyanide mixture. 



913. MALLORY'S Haematoxylin Stains (Journ. Exper. Med., v, 

 1900, p. 19). Tissues to be fixed, mordanted and cut as directed 

 under MALLORY, 911. The sections are put for a quarter of an 

 hour into -5 per cent, solution of potassium permanganate, washed 

 and put for another quarter of an hour into 1 per cent, solution 

 of oxalic acid, well washed and stained for twelve to twenty-four 

 hours or more in MALLORY'S phosphotungstic hcematoxylin. Wash, 

 dehydrate in 95 per cent, alcohol, clear with origanum oil, mount 

 in xylol-balsam. Axis cylinders and nerve cells pink, neuroglia 

 blue. To get a more isolated stain of neuroglia, the sections should 

 be brought for five to twenty minutes, after staining, into a 30 per 

 cent, alcoholic solution of iron sesquichloride. Neuroglia and fibrin 

 blue, the rest colourless. 



MALLORY'S phospho-molybdic hwmatoxylin may also be used for 

 the stain, but it is less elective. 



914. ANGLADE and MOREL'S Victoria Blue Method (Rev. N enrol., 

 ix, 1901, p. 157). Harden in a mixture of. 3 parts of liquid of Fol. 



31-2 



