486 NEUROGLIA AND SENSE ORGANS. 



plished in a few seconds. Sections are finally collected and washed in 

 slightly warmed and repeatedly changed xylol, and mounted in xylol - 

 damar without a cover. 



KULTSCHITZKY Rubin's Method (Anat. Anz., viii, 1893, p. 357) is no 

 longer used. For the slight modification of this method of POPOW, see 

 ZtscJir. wiss. Mikr., xiii, 1896, p. 358, and for that of BURCIIARDT, La 

 Cellule, xii, 1897, p. 364. 



The method of YAMAGIWA (Virchow's Arch., clx, 1900, p. 358) is also 

 no longer used. 



918. Methods for Protoplasmic Neuroglia and Neuroglia Granules. 



OPPENHEIM (N enrol. Centrbl., xxvii, 1908, p. 643) mordants sections 

 made from frozen formalin material with Weigert's copper acetate- 

 chromium fluoride mixture and then stains them with Weigert's iron 

 hsematoxylin prepared without hydrochloric acid. An important 

 point of this method is that the material and the sections should 

 not have been treated with alcohol before staining. 



EISATH (Monatschr. Psych. Neurol, xx, 1906, p. 3 ; Arch. Psych. 

 Nervenkmnkh., xlviii, 1911, p. 897) fixes large pieces in a modified 

 Orth's formol-Miiller mixture consisting of water 1000 c.c., potas- 

 sium bichromate 25 grms., sodium sulphate 15 grms., and formalin 

 150 c.c., to be added at the moment of using the mixture. After 

 about four weeks the tissues are ready for being cut without em- 

 bedding, but can be kept for many months, and even years, in 4 per 

 cent, formalin. The sections are collected in 4 per cent, formalin, 

 in which they may be kept until wanted. For the staining the 

 sections are put for thirty seconds in a -2 per cent, solution of sub- 

 limate, well washed in water, and lifted on to the slide, a dilution of 

 an old Mallory's phosphomolybdic-carbolic acid hsematoxylin being 

 poured on them. After a few minutes they are washed with water, 

 differentiated with a mixture of equal parts of 40 per cent, tannic 

 acid, 50 per cent, alcohol, and 20 per cent, pyrogallic acid in 80 

 per cent, alcohol. Wash in alcohol, dehydrate, clear and mount. 



FIEANDT (Arch. mikr. Anat., Ixxvi, 191011, p. 125) fixes in 

 Heidenhain's sublimate-trichloracetic mixture, and treats pieces 

 for five^to seven days with 96 per cent, alcohol, to be changed three 

 times during the first twenty-four hours and daily in the following 

 days. After dehydration the pieces are embedded in paraffin as 

 directed by JPrantner. The sections, 3 to 5 ^ thick, are stuck to 

 slides, freed from sublimate by the usual iodine treatment, and then 

 stained for twelve to twenty-four hours with Mallory's phospho- 

 tungstic heematoxylin. Dry with filter paper, differentiate for a 

 few hours in 10 per cent, iron perchloride in absolute alcohol, blot 

 once more with filter paper, wash, dehydrate and mount. 



