CHAPTER XXXV. 497 



with a scalpel and brought into a fixing liquid, and opened therein. 

 With fishes and amphibia also the membranous labyrinth may easily 

 be got away. 



926. Preparation. SCHWALBE (Beitr. z. Phys. (C. Ludwig's 

 Festschr), 1887, p. 200). Fix (cochlea of guinea-pig) for eight to 

 teii hours in " Flemming," wash in water, decalcify (twenty-four 

 hours is enough) in 1 per cent, hydrochloric acid, wash the acid 

 out, dehydrate, and embed in paraffin. 



PRENANT (Intern. Monatsschr. Anat., ix, 1892, p. 28). Open the 

 cochlea in solution of Flemming or of Hermann, and fix therein for 

 four to five hours. Avoid decalcification as far as possible, but if 

 neqessary take 1 per cent, palladium chloride. Make paraffin 

 sections. 



Isolation preparations of the stria vascularis may be made by 

 putting a cochlea for a day into 1 per cent, solution of osmic acid, then 

 for four to five days into 0-1 per cent, solution ; the stria may then 

 be got away whole. 



KATZ (Zeit. wiss. Mik., xxv, 1908, p. Ill) fixes the inner ear, 

 opened, for one or two hours in 30 c.c. of 0*5 per cent, osmic acid with 

 5 drops of acetic acid, then adds 10 drops of acetic acid and 

 60 c.c. of chromic acid (or platinum chloride) of 0-5 per cent, and 

 leaves it for four days therein. He then rinses, puts for twelve to 

 twenty-four hours into pyroligneous acid or pyrogallol or tannin 

 solution, decalcifies (not necessary for mice) in 200 parts of water 

 with 1 of chromic acid and 4 to 10 of nitric or hydrochloric acid, and 

 embeds in celloidin or sometimes paraffin. 



Similarly WITTMAACK, see 799. 



BIELSCHOWSKY and BRUEHL (Arch. mik. Anat., Ixxi, 1908, p. 27) 

 fix the petrous in formol of 20 per cent., decalcify it in nitric acid of 

 5 per cent., wash this out, and put back for a few days into the 

 formol, cut by the freezing method, and silver by the neurofibril 

 method ( 840 twenty-four hours in nitrate of 4 per cent., but only a 

 few minutes in the oxide bath). 



Similarly MULLENIX (Bull. Mus. Comp. Zool. Harvard Coll., liii, 

 1909, p. 215). 



STEIN (Anat. Am., xvii, 1900, p. 398) decalcifies in celloidin by the 

 method of ROUSSEAU. So also KISHI (Arch. mik. Anat., lix, 1902, 

 p. 173). 



For staining, RANVIER (Traite, p. 991) employs his gold and formic 

 acid method. 



The bichromate and silver method of GOLGI may be employed 

 with/ce tal or new-born subj ects . The methylen blue intra vitam method 



M. 



32 



