510 METHODS FOR INVERTEBRATES. 



JAQUET (Bib. Anat., iii, 1895, p. 32) kills Lumbricus in extension in 

 1 part of nitric acid to 125 of water. 



COLLIN (Zeit. wiss. Zool., xlvi, 1888, p. 474) puts Criodrilus 

 lacuum into a closed vessel.with a little water, and hangs up in it a 

 strip of blotting-paper soaked in chloroform. KUKENTHAL (Die 

 mik. Technik, 1885 ; Zeit. wiss. Mik., 1886, p. 61) puts Annelids into 

 a glass cylinder filled with, water to the height of 10 cm., and then 

 pours 70 per cent, alcohol to a depth of 1 to 2 cm. on to the water. 

 For Opheliadae he also employs 0-1 per cent, of chloral hydrate in 

 sea water. 



Many -marine Chaetopoda may be successfully narcotised (Lo 

 BIANCO) in sea water containing 5 per cent, of alcohol, or by means 

 of the mixture, 18. 



The Polychceta sedenlaria may sometimes be satisfactorily fixed 

 by bringing them rapidly into corrosive sublimate. Cold, not hot, 

 solutions should be taken, as heat frequently shrivels up the branchiae. 

 Eunice and Onuphis may be treated in the same way. 



Lo BIANCO advises killing Cheetopteridse, Sternaspidse, Spiro- 

 graphis, Protula, by putting them for half an hour into 1 per cent, 

 chromic acid. Some of the sedentaria may be got protruded from 

 their tubes by leaving them for some hours in 0-1 per cent, chloral 

 hydrate in sea water. 



For EISIG'S methods for Capitellidse see Fauna u. Flora Golf. 

 Neapel, xvi, 1887, p. 295. 



See also 14 (lemon juice), and the methods 20 to 26, 39 and 49. 



956. Blood-vessels of Annelids (KUKENTHAL, Zeit. wiss. Mik., 

 1886, p. 61). The animals should be laid open and put for two or 

 three hours into aqua regia (4 parts of nitric acid to 2 of hydrochloric 

 acid). Vessels black, on a yellow ground. 



BERGH (Anat. Hefie, xlv, 1900, p. 392, and xlix, 1900, p. 599) 

 puts small Annelids for a week or more into equal parts of 1 per cent, 

 nitric acid and 1 per cent, nitrate of silver, or into 50 parts of nitrate, 

 25 of formic acid, and 25 of water, dissects out the organs and exposes 

 to light. Marine forms may be treated by HARMER'S process. 



957. Nerves of Annelids. Note the methylen blue method and 

 the bichromate of silver method of Golgi (the rapid method). For 

 the latter see v. LENHOSSEK (Arch. mik. Anat., xxxix, p. 102). 



LANGDON (Journ. Comp. Neur., x, 1900, p. 4) injects strong 

 solution of methylen blue into the body cavity of Nereis, and puts 

 the animal for some hours into sea water in the dark, fixes the stain 

 by Bethe's method, and makes paraffin sections. 



