546 METHODS FOR INVERTEBRATES. 



(4) Wash thoroughly in tap- water. 



(5) Stain and warm gently : (a) 5 per cent, solution of silver nitrate 

 in water ; (6) ammonia. Add the ammonia until the precipitate redis- 

 solves in excess of ammonia. Then add more silver nitrate drop by drop 

 until the precipitate reappears and remains constant. 



(6) Wash thoroughly in tap-water. 



(7) Dry rapidly in air. 



1030. Sphserozoa. BRANDT (Fauna u. Flora Golf. Neapel, xiii, 

 1885, p. 7) fixes with chromic acid of 0-5 per cent, to 1 per cent, 

 (half an hour to an hour), or with a mixture of equal volumes of sea 

 water and 70 per cent, alcohol with a little tincture of iodine for a 

 quarter to half an hour, or with a 5 to 15 per cent, solution of 

 sublimate in sea water. 



KARAWAIEW (Zool Anz., xviii, 1895, p. 286) fixes Aulacantha for 

 twenty-four hours in equal parts of strong liquid of Flemming and 

 acetic acid, and hardens for several days in pure liquid of Flemming. 



See also Lo BIANCO, 1021. 



1031. Sporozoa. WASIELEWSKI (Sporozoenkunde, Jena, 1896, 

 p. 153) studies them living in their natural medium, or in normal 

 salt solution, or in a medium composed of 20 parts white of egg, 

 200 of water, and 1 of common salt. He fixes Gregarinse and 

 Coccidia with osmic acid, sublimate, or picro-sulphuric acid, and 

 Myxosporidia with liquid of Flemming. 



SCHAUDINN (Zool. Jahrb., Abth. Anat., xiii, 1900, p. 197) fixes 

 Coccidia with a mixture of 2 parts of saturated aqueous sublimate 

 and 1 of absolute alcohol, with, if desired, a trace of acetic acid. 



STEMPELL (Arch. ProtistenL, xvi, 1909, p. 389) fixes caterpillars 

 infected with Nosema in 2 parts of saturated sublimate with 1 of 

 alcohol and a little acetic acid, and stains sections for as much as 

 four days in GIEMSA'S mixture, rinses with alcohol and passes 

 through xylol into balsam. 



LEGER (ibid., iii, 1904, p. 311) fixes cysts for a minute in " acetic 

 sublimate," puts for a minute into absolute alcohol, and stains as a 

 smear with heemalum or iron hsematoxylin. 



BRASIL (Arch. Zool. Exper., 4, iv, 1905, p. 74) fixes them for 

 twenty-four hours in a mixture of 1 grm. picric acid, 15 c.c. acetic 

 acid, 60 c.c. formol and 150 c.c. alcohol of 80 per cent., and stains 

 paraffin sections in iron hsematoxylin followed by eosin and orange G., 

 or Lichtgriin and picric acid. 



1032. Hsematozoa. GRASSI (Att. Accad. Lincei, iii, 1900, p. 357) 

 demonstrates the Malaria-parasites in the intestine, body-cavity 

 and salivary glands of Anopheles by treating them with normal salt 



