CKLL-FORMATION. 97 



veloped cell in germination. The examples just given of 

 free cell-formation, without visible nucleus., agree with each 

 other in the point that the new cells first appear as little 

 spherical, homogeneous portions of the contents of the 

 parent-cell, around which no membrane, but merely a 

 definite outline, can be perceived. They are, like the 

 contents of the parent-cell, coloured (green or red) or 

 colourless. Not until they acquire greater size, and in 

 most cases, only at the time when they become granular, 

 does the membrane become distinctly visible. A nucleus 

 is never seen in the earlier stages, and appears sub- 

 sequently only in isolated cases. 



I have made a second series of observations on free 

 cell-formation without visible nucleus in Alga3 and aquatic 

 Fungi, especially in Bryopsis, Codium, Anadyomene, 

 Acetabularia, Dasycladus, Conferva glomerata (lacustris 

 et marina), and AcJdya prolifera. I regard it as an ab- 

 normal cell-formation, because it mostly occurs in older 

 cells, and frequently simultaneously with the death of 

 the rest of the cell-contents, or precedes this. If this 

 mode of cell-formation occurs in healthy cells, containing, 

 in addition to colourless^ transparent contents, and homo- 

 geneous and granular mucilage, which is especially mani- 

 fest as the mucilaginous layer investing the internal surface 

 of the wall, also chlorophyll-globules and starch-granules, 

 which lie principally in the mucilaginous layer, at first 

 we see minute, homogeneous, colourless mucilage-globules, 

 the diameter of which is about '002-'004 of a line. They 

 become larger, finely granular, acquire a greenish colour, 

 and a membrane gradually becomes visible upon them. 

 Subsequently they acquire very various sizes ('OlO-'OGO'"), 

 and contents which vary much in quantity, since the 

 chlorophyll sometimes lies upon the wall in smallish 

 quantity, and sometimes gives the cell a deep green aspect 

 by its great abundance. 



In the above cases the cells originate and are perfected, 

 without immediately effecting any essential change in the 

 contents of the parent-cell. But if the parent-cells have 



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