EXTENSION OF THE GERM THEORY 391 



kept at the constant temperature of about 35 C. The next 

 day, the culture fluid had become cloudy and contained a 

 single organism, consisting of small spherical points ar- 

 ranged in pairs, sometimes in fours, but often in irregular 

 masses. Two fluids were preferred in these experiments 

 chicken and yeast bouillon. According as one or the other 

 was used, appearances varied a little. These should be de- 

 scribed. With the yeast water, the pairs of minute granules 

 are distributed throughout the liquid, which is uniformly 

 clouded. But with the chicken bouillon, the granules are 

 collected in little masses which line the walls and bottom 

 of the flasks while the body of the fluid remains clear, un- 

 less it be shaken: in this case it becomes uniformly clouded 

 by the breaking up of the small masses from the walls of 

 the flasks. 



Second observation. On the tenth of June a new fu- 

 runcle made its appearance on the right thigh of the same 

 person. Pus could not yet be seen under the skin, but this 

 was already thickened and red over a surface the size of 

 a franc. The inflamed part was washed with alcohol, and 

 dried with blotting paper passed through the flame of an 

 alcohol lamp. A puncture at the thickened portion enabled 

 us to secure a small amount of lymph mixed with blood, 

 which was sowed at the same time as some blood taken 

 from the finger of the hand. The following days, the 

 blood from the finger remained absolutely sterile: but that 

 obtained from the center of tjie forming furuncle gave an 

 abundant growth of the same small organism as before. 



Third observation. The fourteenth of June, a new fu- 

 runcle appeared on the neck of the same person. The same 

 examination, the same result, that is to say the develop- 

 ment of the microscopic organism previously described and 

 complete sterility of the blood of the general circulation, 

 taken this time at the base of the furuncle outside of the 

 inflamed area. 



At the time of making these observations I spoke of them 

 to Dr. Maurice Reynaud, who was good enough to send me 

 a patient who had had furuncles for more than three months. 

 On June thirteenth I made cultures of the pus from a fu- 

 runcle of this man. The next day there was a general 



