Doubtful Species. 



Separate by careful examination of the wing markings. ( See 

 coloured plates and descriptions.) 



The following, which are described as Indian species by Mr. 

 Theobald, are not included in the synoptic table : 



(1.) Anopheles (Myzomyia) leptomeres. 

 (2 ) ,, (Myzorhynchus) vanus. 



(3.) minutus. 



(4.) (Aldrichia) error. 



Some remarks regarding these species will be found in Part II. 



The examinat-on and identification oflarvce. The larvae of 

 mosquitoes may be examined when alive or when killed and mounted 

 on slides. In order to examine a larva when it is alive it is caught 

 in a small spoon care being taken not to injure it and transferred 

 with a drop of water on to a glass slide. A cover glass is then 

 dropped very gently on to it. This has the effect of preventing 

 the larva from continually wriggling out of the field of the 

 microscope, and, if carefully done, it does not break any of the 

 larval hairs or injure it in any way. 



Permanent specimens of larvae may be obtained either by 

 mounting them simply in a solution of formalin in hollow ground 

 glass slides, applying a cover glass and ringing it with thick Canada 

 balsam, or by the following more permanent method, first 

 recommended by Dr. Christophers. 



(a) Kill the larva by immersing it in strong formalin solution, 

 and allow it to remain in this solution for at least 

 twelve hours. 



(6) Immerse the larva after treatment in the formalin solution, 

 in absolute alcohol for fifteen minutes. 



(c) Immerse it in oil of cloves. 



(d) Clear with xylol and mount on an ordinary flat slide under 



a cover glass in xylol balsam. 



During the above process very great care must be taken to 

 handle the larvae gently so as not to break any of the finer hairs. 

 They are best removed from one dish to another by lifting them 

 up very gently on the edge of a piece of stiff paper. 



Another plan, which shows the palmate hairs and some other 

 characters of the larvae exceedingly well, consists in mounting the 



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