14 PRACTICAL BACTERIOLOGY 



II. The following articles are also placed on the table : 



1. A large glass dish, 6 inches by 7 inches, with a cover, con- 



taining Disinfecting Solution (see 66), for the temporary 

 disposal of old cultures, virulent material, etc. 



2. A small glass dish, 3| inches by 2 inches, containing Cleansing 



Solution (see 65), for the temporary disposal of dirty 

 slides and cover-glasses. 



3. A covered glass dish, containing vaseline and a small camePs- 



hair brush, for preparing hanging-drop cultures. 



4. One large test-tube stand. 



5. One small test-tube stand. 



6. One small filter stand. 



7. One bottle of Canada balsam dissolved in xylol. 



8. One small bottle of immersion oil. 



9. Several ordinary glass tumblers, some of which are used for 



holding platinum needles, clean water, scissors, forceps, 

 glass rods, etc. A drawer is necessary in the table to 

 hold clean cover-glasses, slides, test papers, filter paper, 

 watch glasses, staining dishes, etc., when not in actual 

 use. 



The following stains and reagents are placed in the aforementioned 

 rack for ordinary use : 



1. Loffler's solution of methylene blue. 



2. Watery alcoholic solution of gentian violet. 



3. Watery alcoholic solution of fuchsin. 



4. ZiehPs carbol fuchsin, or Ehrlich's anilin water fuchsin. 



5. Ehrlich's anilin water gentian violet. 



6. Roux^s double stain. 



7. 2 per cent, acetic acid in water. 



8. 3 per cent, hydrochloric acid alcohol. 



III. Methylene blue and gentian violet give excellent staining 

 results, and are useful stains for most bacteria, while fuchsin is one of 

 the simplest and most rapid stains to manipulate. ZiehPs carbol 

 fuchsin is the most stable preparation of fuchsin used in staining 

 Tubercle bacilli. EhrlicK's anilin water fuchsin and gentian violet 

 give excellent staining results, but are very unstable, soon decomposing, 

 requiring to be freshly prepared every three weeks. Roux's double 

 stain is specially adapted for the detection of Diphtheria bacilli, inas- 

 much as the bacilli stain more readily and with greater intensity than 

 any of the other organisms usually found associated with them. (For 

 the preparation of the above stains and reagents see 49-60.) 



