20 PRACTICAL BACTERIOLOGY 



3. Bacillus of Leprosy. 



4. Bacillus of Mouse Septicaemia. | Different varieties of 



5. Bacillus of Rouget, or Swine Erysipelas, j the same species. 



6. Bacillus of Tetanus. 



7. Streptococci of Pyaemia and Erysipelas. 



8. Staphylococcus pyogenes aureus. 



9. Staphylococcus pyogenes citreus. 



10. Staphylococcus pyogenes flavus. 



11. Staphylococcus pyogenes albus. 



12. Micrococcus tetragenus. 



13. Diplococcus pneumoniae, ' Fraenkel.' 



14. Actinomyces hominum.* 



15. Actinomyces bovis.* 



16. Actinomyces musculorum suis. 



17. Botryomyces ' Bollinger. 1 ( 



18. Discomyces ' Rivolta.' f 



19. Botryococcus ascoformans ' Kitt.' ( 



20. Bacillus of Diphtheria. ^ Only under certain 

 Bacillus of Black Leg, or V conditions and 



' Symptomatic Anthrax.' J circumstances. 



21. The Lactic Acid Bacillus of Hueppe. 



XII. THE 'CLADIUS' CONTRAST METHOD FOR 

 COVER-GLASS SPECIMENS. 



By adding J per cent, solution of picric acid in water to 1 

 per cent, solution of methyl-violet in water a blue indigo colour is 

 the result, insoluble in water, very soluble in alcohol, chloroform, 

 anilin, and clove oils. 



METHOD OF PROCEDURE. 



1. Place the material on a cover-glass and air dry. 



2. Pass three times through the Bunsen flame. 



3. Stain one minute in a 1 per cent, watery solution of methyl- 

 violet. 



4. Wash in water and dry with filter paper. 



5. Pass through J per cent, picric acid solution (see 61) one 

 minute. 



6. Wash in water ; dry with filter paper. 



* At stage 4, during decolorization with alcohol the cover-glass must be kept 

 moving, otherwise, if left still, the Actinomyces are almost entirely decolorized. 

 t Named according to investigators. 



