BACTERIOLOGICAL TECHNIQUE 27 



the solution, and heat gently do not boil leaving the specimen in 

 the solution ten to fifteen minutes. 



5. Wash the preparation in the ordinary manner with water. 

 Examine in water, and if satisfactory mount in xylol balsam. The 

 advantage of examining the specimen in water is that the flagella 

 appear more distinct than in balsam, and if too faintly stained, the 

 specimen can again be placed in the orcein solution, and the process 

 rt 



XXV. THE FLAGELLA OF THE FOLLOWING BACTERIA 

 STAIN BY THE ABOVE METHOD. 



1. Spirillum choleras Asiaticae. 



2. Bacillus typhi abdominalis. 



3. Bacillus coli communis. 



4. Klein's Bacillus of Swine Fever. 



5. Bacillus subtilis. 



6. Bacillus violaceus. 



7. Bacillus fluorescens liquefaciens. 



8. Bacillus prodigiosus. 



9. Proteus vulgaris. 



10. Vibrio Finkler-Prior. 



11. Vibrio Metschnikoff. 



12. Vibrio aquatalis. 



13. Vibrio Berolinensis. 



14. Vibrio Rugula. 



15. Bacteria in hay infusion. 



16. Bacteria in oat-straw infusion. 



17. Infusoria and various bacteria in canal water. 



18. Bacillus tetani. 



19. Bacillus oedematis maligni. 



20. Bacillus of Symtomatic Anthrax. 



XXVI. METHODS OF STAINING SPORES. 



Endospores possess a firm membrane or capsule of great resistance 

 towards staining reagents, and can only be stained with certainty on 

 cover-glass specimens. 



ORDINARY METHOD. 



1. Prepare the specimen in the ordinary way (see 8), air dry, 

 and fix in the flame by the ordinary method. 



2. Float the cover-glass, preparation side downwards, on a watch 



