PRACTICAL BACTERIOLOGY 



XXXIV. BEFORE FREEZING. 



All traces of alcohol must be removed from the hardened tissue as 

 follows : 



1. Place in a 1 per cent, solution of 40 per cent, formalin in water 

 for two hours. Pieces of fresh tissue are prepared in a 

 similar manner. 





FIG. 8. Jung's Students' Microtome. 



A piece of Joseph's paper or ordinary lens paper is laid on the 

 freezing plate of the microtome, the tissue laid on top of 

 it, and frozen with the ether spray. When hard it is cut 

 into sections, and the cut sections placed in water. 



