SUMMARY OF CONTENTS 



PAGE 



INTRODUCTION, ....... 1 



THE CLASSIFICATION AND MORPHOLOGY OF BACTERIA 



Flagella or Motile Organs, Dimensions, Multiplication, and 



Spore Formation, ..... 3-7 



METHODS OF STERILIZATION 



Ordinary Methods, ...... 7-1 1 



Tyndall's Discontinuous Method, . . . . 11 



Sterilization of Post-mortem Instruments, . . . 12 



PART I. 



PRINCIPLES OF BACTERIOLOGICAL TECHNIQUE 



The Working Table, . . . . .13 



General Methods of Bacteriological Investigation, . . 15 



Methods of Preparing and Staining Cover-glass Specimens, 



The Hanging-drop, etc. .... 15-30 



Methods of examining Sections of Organs and Tissues for 

 the detection of Bacteria ; Embedding ; Section 

 Cutting ; and Staining Methods, . . . 30-40 



Stains, Mordants, and Special Reagents, . . . 40-46 



PART II. 



THE PREPARATION OF NUTRIENT MEDIA 



Bouillon Media, ...... 47 



Potato . . . . . .48 



Peptone '.'/ .50 



Milk . . . .51 



Egg 51 



Gelatine . . . . .52 



Agar-Agar . . . 53 



Blood Serum . . . . .55 



Special . . . . .57 



