36 PRACTICAL BACTERIOLOGY 



XL. EHRLICtTS METHOD OF STAINING TUBERCLE 

 BACILLI IN SECTIONS OF TISSUE, ORGANS, &c. 



(a) ALCOHOL SECTIONS. 



1. Place sections in Ehrlich's anilin water fuchsin* (see 55), and 

 set the glass dish in the incubator at 37 C. for one hour and a half, 

 and at room temperature twenty-four hours. 



2. Wash in water five minutes. 



3. Into 3 per cent. HC1. alcohol one minute, and move round, not 

 allowed to simply lie in the alcohol. 



4. Wash in water. 



5. Contrast stain with methylene blue or Bismarck brown. 



6. Wash in water. 



7. Into alcohol to remove water. 



8. Into xylol. 



9. Mount in xylol balsam. 



*Ehrlich's anilin water gentian violet can also be used, or Ziehl's carbo 

 fuchsin. 



(b) FROZEN SECTIONS. 



1. Transfer the sections from the salt solution into 80 per cent, 

 alcohol for five minutes to harden ; or, 



2. Harden in corrosive sublimate, 1 to 1000-1500 for one-half 

 to one hour, and wash in water, and proceed as above at process 

 No. 1. 



XLI. LOFFLER'S UNIVERSAL METHOD OF STAINING 



SECTIONS. 



1. Bring the sections out of alcohol into Loffler's methylene blue 

 (see 54) for five to thirty minutes. 



2. Into 1 per cent acetic acid. 



3. Into absolute alcohol. 



4. Into xylol. 



5. Mount in xylol balsam. 



The amount of time the section remains in the acetic acid solution 

 will depend on the variety of organism the operator is working with 

 By this method 



Bacilli are stained = dark black-blue. 

 Cellular constituents = blue. 

 The Protoplasm = light blue. 

 This method can be employed for almost all bacteria. 



