BACTERIOLOGICAL TECHNIQUE 37 



XLII. WEIGERTS METHOD FOR FIBRIN OR 

 BACTERIA IN SECTIONS. 



1. Harden section in alcohol. 



2. Stain in Ehrlich's anilin water gentian violet (see 55) five 

 to fifteen minutes. 



3. Wash the section in 6 per cent, solution of chloride of sodium. 



4. Dry the section with filter-paper. 



5. Place in a 1 or 2 per cent, watery solution of iodide of potash 

 for two or three minutes. 



6. Dry with filter-paper. 



7. Decolorize in the following solution : 



Anilin oil, . . . 2 c. c. 

 Xylol, . . . 1 c.c. 



8. Place the section in xylol to remove the anilin oil. 



9. Mount in xylol balsam. 



XLIII. LOFFLER'S METHOD OF STAINING THE 

 GLANDERS BACILLUS IN SECTIONS. 



1. Stain the section in Loffler's methylene blue (see 54) for a 

 few minutes. 



2. Place the section in the following solution : 



Aqua distilli, . . . . 10 c. c. 

 Coned. Sulphuric acid, . . 2 drops. 

 5 per cent. sol. of Oxalic acid, . 1 drop, 

 for five seconds. 



3. Decolorize and dehydrate in absolute alcohol. 



4. Place the section in xylol. 



5. Mount in xylol balsam. 



XLIV. ANOTHER METHOD OF STAINING THE 

 GLANDERS BACILLUS IN SECTIONS OF TISSUES. 



1. Transfer the sections from alcohol to distilled water. 



2. Transfer from the water to a slide, and dry thoroughly with 

 filter-paper. 



3. Stain with a few drops of the following stain for thirty 

 minutes : 



Carbol. fuchsin, . . 10 c. c. 

 Distilled water, . . 100 c. c. 



