STAINS 41 



Or (3) fill an ordinary test-tube three-quarters full of distilled 

 water, and add enough of the concentrated alcoholic or watery solution 

 of the dye, until you can just see through the solution, which is ready 

 for use. 



LI. SPECIAL STAINS FOR COVER-GLASS SPECIMENS 

 AND SECTIONS OF TISSUES. 



ZiehPs carbol. fuchsin. 



Fuchsin . . . . 1*0 gramme. 



Acid, carbolic . . . 5*0 grammes. 



Alcohol .... lO'O c. cm. 



Aquadistilli . . . . 100 



LII. GABBETS SOLUTION. 



Methylene blue . . . 1 to 2 grammes. 



Aqua distilli . . . 75 c. cm. 



Concentrated sulphuric acid . 25 



This solution is used in the Ziehl Gabbet method for demon- 

 strating the presence of the bacillus of tuberculosis ; the acid 

 decolorizes, whilst the methylene blue acts as a contrast stain, and 

 is of especial value owing to the simplicity and rapidity of its action. 



LIIL ROUX'S DOUBLE STAIN. 



Dahlia or gentian violet . . *5 grammes. 



Methyl green . . . 1*5 



Aqua distilli . . . 200 c. cm. 



METHOD FOR COVER-GLASSES. 



1. Air dry and fix cover-glass. 



2. Stain from five to ten seconds. 



3. Wash in water. 



4. Dry and mount in Canada balsam dissolved in xylol. Sections 

 remain in this stain twelve hours, then washed, dehydrated, and 

 mounted. This stain is, as already mentioned, of especial value in the 

 examination of diphtheritic specimens. 



LIV. LOFFLER'S METHYLENE BLUE. 



Concentrated alcoholic Solution of methylene blue, 30 c. cm. 



Watery solution of caustic potash (1 : 10,000) 100 



This stain is specially adapted for staining the glanders bacillus. 



