42 PRACTICAL BACTERIOLOGY 



LV. EHRLICITS ANILIN WATER, GENTIAN VIOLET, 

 FUCHSIN, OR METHYL VIOLET. 



Anilin oil . 4c. cm. ) rr n. . , ... 



T.. ..,,-, ^rvr, r Inis is known as amlm water. 



Distilled water 100 J 



Shake the above well together, filter, and add lie. cm. of a con- 

 centrated alcoholic solution, gentian violet, methyl violet, or fuchsin, 

 whichever stain is required ; shake the mixture, and set aside from 

 twelve to twenty-four hours before using. 



These stains yield good results in staining many forms of 

 organisms, especially tubercle bacilli, and in conjunction with Gram's 

 method ; but they have one drawback, they are very unstable, and 

 soon decompose, requiring to be renewed every three weeks. 



LVI. KUHNE'S METHYLENE BLUE. 



Methylene blue . . . 1-5 grammes. 



Absolute alcohol . . . lO'O 



5 per cent, acid carbolic in water . 100*0 

 Mix the methylene blue and the alcohol, rub in a mortar until the 



methylene blue is thoroughly dissolved, and then add the 5 per cent. 



solution of carbolic acid. 



LVII. CHLOROFORM FUCHSIN SOLUTION. 



(1) One or two crystals of fuchsin are dissolved in chloroform 

 2 to 3 c. cm. 



Or (2) concentrated alcoholic solution of fuchsin, three or four 

 drops chloroform 2 to 3 c. cm. 



Arenas method of staining tubercle bacilli in fatty substances, milk, 

 etc., etc. 



LVIII. NEISSER'S STAINS FOR DIFFERENTIAL 

 DIAGNOSIS OF DIPHTHERIA. 



I. 



Methylene blue ... 1 gramme. 



96 per cent, alcohol . . . 20 c. cm. 

 When dissolved, add 



Aqua distilli .... 950 c. cm. 



Glacial acetic acid . . . 50 



II. 



Vesuvin . . . .8 grammes. 



Aqua distilli boiled . . . lOOO'O c. cm. 



