44 PRACTICAL BACTERIOLOGY 



LXIII. BUNGE'S MORDANT. 



Ferric chloride B.P. solution diluted 1 to 20 of 



water, . . . . 25 c. cm. 



Saturated aqueous solution of tannin, . 75 



N.B. Recent researches by Gunther, Fischer, and Lehman, show 

 that the addition of alkalis or of acids, according to Loffler, are 

 superfluous, as also the hydrogen per oxide solution added by Bunge 

 to his mordant. 



LXIV. SOLUTION FOR CLEANSING AND DISINFECTING 

 FOR COVER-GLASSES. 



Newly purchased cover-glasses are placed in a solution of nitric 

 acid and water for twenty-four hours ; removed, washed in water, and 

 kept until required in alcohol. The advantages of really clean cover- 

 glasses and slides are appreciated by experienced microscopists. 



LXV. SOLUTION FOR DIRTY COVER-GLASSES, SLIDES, 

 AND OTHER GLASSWARE. 



Water . . . . 50 c. cm. 



Methylated alcohol . . 45 



Strong ammonia . . . 5 



LXVI. DISINFECTING SOLUTION FOR POTATOES, 



HANDS, &c. 



Bichloride of mercury ... 1 gramme. 



Water ..... 1000 c. cm. 

 Hydrochloric acid, ' strong ' ' ' . . 5 



LXVII. LOFFLEITS METHOD FOR CLEANSING 

 COVER-GLASSES. 



1. Warm the cover-glasses for some time in concentrated sul- 

 phuric acid. 



2. Rinse in water. 



3. Place until wanted in a mixture of equal parts of alcohol and 

 ammonia. 



4. Before use, dry with a cloth, from which all fat has been 

 extracted. 



