52 PRACTICAL BACTERIOLOGY 



3. Make an opening at one end of the egg with a hot needle and 

 inoculate the egg. 



4. Close the opening in the egg with a small piece of silk paper, 

 and then apply a coating of collodion. 



LXXXVII. GUNTHER'S METHOD. 



1. Wash one end of an egg with soap and water and a brush. 

 Sterilize one end of the egg with the flat surface of a clean hot 

 potato-knife. 



3. Heat a steel needle, and when cool, make an opening in the 

 sterilized spot, large enough to admit the platinum needle with 

 which the egg is inoculated. 



4. Close with sterile paper and collodion, or with hot sealing-wax. 



LXXXVIII. EGG ALBUMEN METHOD. 



Take the white of an egg and distribute it in tubes, slant, and 

 coagulate as with blood serum (see 99.) 



GELATINE MEDIA. 

 LXXXIX. NUTRIENT GELATINE. 



1. Make 1 litre of meat infusion (see Bouillon, processes Nos. 1 

 and 2, 73). 



2. Gelatine . . . .100 grammes. 

 Peptone siccum . . . . 10 

 Common salt . . . . 5 



are placed in a glass boiling flask, and the meat infusion added last. 



3. The gelatine and peptone are thoroughly dissolved in a water- 

 bath, between 40 and 50 C., or directly over the flame, using an 

 ordinary enamel saucepan, with several pieces of wire gauze or a sheet 

 of asbestos intervening between the flame and the pan. 



4. Test the reaction, make alkaline with a concentrated solution 

 of sodium carbonate (the same caution is requisite as described at 

 Bouillon, process No. 5, 73), and add a fresh egg. 



5. Place in the steam sterilizer for one and a quarter hours (too 

 long boiling lowers the solidifying point of the gelatine), remove and 

 filter through two thicknesses of filter paper. (Fold the filter paper 

 with a sharp point pushed well down into the neck of the glass funnel^ 



