MEDIA FOR NITRIFYING BACTERIA 57 



ligature. The clamp is then removed, when the bulb is quickly filled 

 with blood. Replace the clamp, remove the bulb, and seal in the gas 

 flame. The loose ligature is now tightened and the wound closed. 

 The glass bulb is put in a cool place until coagulation has occurred. 

 The serum is withdrawn with a sterile pipette. 



CIII. MEDIA FOR MOULD FUNGI. 



1. Place some dry bread, finely grated, into test-tubes about 1| 

 inch high in each tube, or into Erlenmeyer flasks, about 1 inch in 

 each. 



2. Add enough water to convert the bread into a paste, and close 

 the tubes or flasks with cotton plugs. 



3. Sterilize in the steam sterilizer for fifteen minutes on each of 

 three successive days. 



The bread paste having an acid reaction cannot be employed for 

 the cultivation of bacteria. 



CIV. SABOURAUD^S MEDIUM FOR FAVUS, ETC. 



Peptone . . . 0'8 gramme. 



Mannite . . . 3'80 grammes. 



Aqua distillata . . lOO'O c.c. 



Agar-agar . . . 1*40 gramme. 



Prepare in the ordinary manner, make slightly alkaline, fill in 

 tubes, and sterilize fifteen minutes on each of three successive days. 



MEDIA FOR NITRIFYING BACTERIA. 



CV. WINOGRADSKY'S SOLUTION. 



Ammonia sulphate . . 1 gramme. 



Potassium phosphate . . 1 



Pure water . . . 1000 c.c. 



Place 100 c.c. of the above solution in each flask, and add to each 



0*5 to 1-0 gramme of basic magnesium carbonate, suspended in 



distilled water, and sterilize by boiling. 



CVI. PREPARATION OF TUBES, FLASKS, ETC., FOR 

 THE PRESERVATION OF CULTURE MEDIA. 



Both new tubes and those previously used should be boiled for an 

 hour in a 2 to 3 per cent, solution of common soda, and carefully 



