126 SPECIAL BACTERIOLOGY 



of the middle puncture, a film developing on the surface ; old cultures 

 gi ve off a slight odour. 



On the surface of Agar at 37 C. a copious grey transparent coating 

 o ccurs, and a slight odour is given off. 



On Potatoes at 37 C. in two days a somewhat copious greyish-white 

 shiny coating develops. 



Bouillon cultures exhibit strongly-marked cloudiness throughout the 

 medium. 



Grape Sugar Bouillon exhibits the same changes as occur with the 

 Bacterium coli commune ; the medium becomes strongly acid, and gas is 

 formed, sometimes CO 2 and sometimes a combustible gas. 



In Milk Sugar Bouillon no acid reaction occurs ; the fluid remains 

 alkaline, but sometimes, although not always, small gas bubbles are found 

 consisting of CO 2 or a combustible gas. 



Milk is not altered in chemical reaction nor coagulated. 



Indol is not formed in twenty-four hours' old cultures at 37 C., and 

 the Nitroso-indol reaction is absolutely negative. 



The Bacillus enteritidis grows excellently under aerobic conditions 

 in the ordinary nutrient media, but only under anaerobic conditions in 

 the presence of grape sugar. 



Pathogenesis. Mice and guinea-pigs are very susceptible to 

 subcutaneous inoculation, while rabbits are less susceptible to infection. 

 Mice die in one to three days, guinea-pigs in about five days, and the 

 inoculated bacteria are again found in the heart's blood and organs of 

 these animals. Mice die in five to eight days when fed with food 

 containing the bacteria, and the specific bacteria are found in their 

 internal organs ; guinea-pigs are also infected by way of the digestive 

 tract. 



Ill man, this bacillus causes gastro-enteric symptoms which may 

 be more or less intense, occasionally causing death, specific bacteria 

 being found in the organs. They appear to be introduced into the 

 intestinal canal with the food ; very probably such meat is obtained 

 from animals in which the Bacillus enteritidis was already present 

 during life. In what animal diseases this bacillus can possibly 

 originate is not known. 



BACILLUS MORBIFICANS BOVIS. 



This organism was found by Basenau in a cow affected with 

 puerperal fever. 



Microscopical Appearances. Rods about the same size as the 

 typhoid bacillus, 0*3 to 0'4 p wide, and 1 to 1*2 //. long, sometimes 

 arranged in pairs. 



Motility. Strongly motile. 



