BACILLUS ORCHITICUS 135 



On Gelatine Plates it forms colonies that resemble old colonies of the 

 bacillus of Asiatic cholera ; liquefaction occurs somewhat rapidly at 22 C. 



On A gar, thick white tufts. 



On Blood Serum an orange-yellow pigment is often formed; the 

 medium is liquefied. 



In Bouillon and Peptone Solution small flakes are formed, the medium 

 very seldom becoming clouded. 



Pathogenesis. Guinea-pigs (male) when inoculated intraperi- 

 toneally with a small quantity of the virus, exhibit swelling of the 

 testicles in forty-eight hours, and generally die in four to five days. 

 The principal lesions are nodules in the mesentery and testicles (rarely 

 in the abdominal organs). Large doses caused death in one to three 

 days with more pronounced changes in the peritoneal cavity. Small 

 doses introduced subcutaneously caused death in one to two days, with 

 an extensive oedema affecting the whole abdominal wall. Guinea-pigs 

 that recovered were found later to be immune to further infection. 

 Mice inoculated subcutaneously with small doses die in four to seven 

 days, an abscess developing, the surrounding tissue being cedematous, 

 and infiltrated with small haemorrhages. The bacilli were only present 

 in the pus from the abscess, and frequently within the leucocytes. 

 Intraperitoneal inoculation produced death in the same time, numerous 

 nodules being formed on the peritoneum ; the liver and spleen being 

 seldom affected. Intrapulmonary injection caused the formation of a 

 watery hsemorrhagic effusion into the serous cavities of the thorax, and 

 the development of numerous grey nodules on the pleurae, associated 

 with small lobular pneumonic centres. 



Rabbits are not so susceptible to infection, while chickens and pigeons 

 are immune. 



Differential Diagnosis. With the Gram method of staining the 

 bacillus of glanders gives negative results, while the Bacillus orchiticus 

 yields positive results. 



EPIZOOTIC LYMPHANGITIS, OR AFRICAN FARCY. 



An organism discribed as the ' Cryptococcus of Rivolta,' a species of 

 micrococcus measuring 3 to 4 /A in diameter, slightly ovoid and somewhat 

 pointed at one of its extremities, has been found in the pus and lesions 

 of this disease by Rivolta and Nocard. It stains by the Gram, Weigert, 

 and Kiihne methods. 



The dimensions and refringence exhibited by the organism are such 

 that it is impossible, even in unstained specimens, to mistake it for any 

 other element (Nocard). 



Ulcers resembling those of acute glanders have been found on the 

 nasal mucosa of animals affected with epizootic lymphangitis. Nocard, 

 however, found the above cryptococcus in these lesions, and thus affirmed 



