ROUX'S METHOD OF IMMUNIZING A HORSE 167 



trikresol which is considered to possess about twice the antiseptic power of 

 carbolic acid, and is only half as poisonous, while in the Pasteur Institute 

 they use a piece of camphor. Serum desiccated in vacua is convenient 

 to send to a distance, and reacquires its preventive properties when 

 dissolved again in eight or ten times its weight of pure water. 



Instructions for the use of Schering's Diphtheria Anti- 

 toxin. A hypodermic syringe is used, holding 10 to 12 c.c., which, 

 before use, must be thoroughly asepticized with alcohol and a 1 per 

 cent, solution of trikresol. 



The injection is made deep into the subcutaneous connective tissue of 

 the skin, the back, between the shoulder blades, or the thighs, being the 

 points of election. Before the injection is made, the skin is scrubbed 

 with soap and water, and then with ether. After the injection the 

 puncture may be immediately sealed with rubber plaster, or cotton and 

 iodoform collodion, to prevent any loss of fluid through the dermal 

 puncture. 



For the immunization of children and adults in families where 

 diphtheria has occurred, isolation being unnecessary, and also for 

 general use among those who have been exposed in epidemics, the dose 

 is as follows : 



Up to two years of age, 0'5 c.c. (8 minims). 



From two to ten years of age, Ic.c. (16 do. ). 

 Over ten years, and for adults, 2 c.c. (32 do. ). 



If in four to six weeks the epidemic has not ended, it is well to 

 repeat the injection. 



For the Cure of Diphtheria extensive practice has shown that a single 

 injection of 5 c.c. (80 minims) of the solution at present supplied is 

 certainly curative if used at the beginning of the disease. In severer cases 

 that have lasted some time, two injections must be given in the course 

 of twelve to twenty-four hours, of 5'20 c.c. (80 minims to 5j fluid 

 drachms), according to the body-weight. In some cases still larger 

 doses have been successfully employed. The further repetition of the 

 injection depends upon the following factors : 



a. The course and increase of the exudation, and the condition of 



the glands. 



b. The course of the temperature. 



c. The condition of the kidneys. 



d. The condition of the pulse and the heart sounds. 



BACILLUS DIPHTHERIA COLUMBARUM. 



Obtained by Loffler in 1884 from the false membranes in the 

 mouths of pigeons. Chickens are also affected. 



