PLEURO-PNEUMONIA BOVIS 201 



On Gelatine Plates. Flat, spread out, bluish, transparent, tabulated 

 colonies. 



In Gelatine Stab Cultures the growth takes place along the inocu- 

 lation track and on the surface of the medium as well ; there is no 

 liquefaction. 



Upon oblique surface Gelatine there is a rapid, pellucid, pearly-white 

 growth, the edges being scalloped. 



On Agar-Agar the edges of the growth are deeply scalloped, the 

 separating lines extending into the body of the cultures. 



On Potato, greyish-white, somewhat elevated colonies develop. 



White of Egg. Clear yellow colonies with slightly raised edges 

 develop. 



Milk is not coagulated. 



Pathogenesis. Mice, rabbits, guinea-pigs, and pigeons inocu- 

 lated subcutaneously with 2 or 3 drops of the serum or culture died 

 in less than forty-eight hours. Intraperitoneal injection caused 

 death in fifteen to eighteen hours with purulent peritonitis. Sheep 

 and calves inoculated subcutaneously or in the trachea with 1 c.c. of 

 culture serosity or virulent pus did not die, but were seriously 

 affected for several days, when they soon regained their normal 

 condition. An eight-month-old calf and a two-year-old ram, 

 inoculated in the right lung with 5 drops of peritoneal pus from a 

 guinea-pig, died in less than forty-eight hours with fibrinous 

 pleurisy and exudative broncho-pneumonia analogous to that 

 observed at the autopsy on the American cattle. The lesions 

 contained numerous quantities of bacteria. Nocard. 



Pigs, dogs, rats, and chickens are immune. A calf and two 

 sheep previously inoculated subcutaneously, then in the trachea, 

 afterwards resisted the effects of intrapulmonary inoculation with 

 10 drops of a virulent culture. 



PLEURO-PNEUMONIA CONTAGIOSA BOVIS. 



Poels and Nolen considered this bovine scourge was caused by a 

 micrococcus, while Arloing isolated a bacillus which he named the 

 Bacillus liquefaciens bovis, but it has been proved that neither of these 

 organisms possess the pathological significance ascribed to them by the 

 above-mentioned investigators. The most recent announcement 

 regarding the cause of this disease was communicated by MM. Nocard 

 and Roux, with the collaboration of MM. Borel, Salimbeni, and 

 Dujardin-Beaumetz, in the Revue Veterinaire, May 1898. 



These investigators placed bouillon previously inseminated with a 



