PATHOGENIC BLASTOMYCETES 251 



SACCHAROMYCES LITOGENES. 



Found in the lymph-glands of an ox affected with carcinoma of 

 the liver. 



Microscopical Appearances. Large and small round cells with a 

 membrane. 



Biological Characters. The growth in the various media is 

 similar to the Saccharomyces neoformans, except on potatoes, when an 

 intense brown coating develops. 



Pathogenesis In guinea-pigs a tumour forms at the point of 

 inoculation and nodules in the organs. The yeast cells in the centre of 

 the nodules very frequently degenerate. 



SACCHAROMYCES NEOFORMANS. 



Found in the juice of fruits. 



Microscopical Appearances. Round or elliptical cells with re- 

 f ractile granules and a double contour, which increase by budding. 



Biological] Characters. On Gelatine Plates, round, cup-shaped, 

 non-liquefying colonies. 



In Gelatine Stab Cultures, a granular growth along the inoculation 

 track. 



On Agar, a dry film develops. 



On Potatoes, a white elevated growth. 



Milk is not coagulated. 



In Sugar Bouillon a sediment forms, and often a film is developed. 



Pathogenesis. In guinea-pigs a tumour forms at the point of 

 inoculation, and nodules develop in the internal organs. 



SACCHAROMYCES SUBCUTANEUS TUMEFACIENS. 



Found in a myxomatous tumour of the upper part of the thigh. 



Microscopical Appearances. Oval or round cells, frequently 

 possessing large transparent capsules. The cells increase by budding. 



Biological Characters. In Gelatine Stab Cultures the develop- 

 ment occurs in small colonies ; it causes no liquefaction of the medium. 

 On Agar, a thick creamy growth. 



On Potatoes, an extensive white coating, which later becomes brown. 

 On Acid Beer-wort Agar, a brown coating. 

 In Alkaline Bouillon, a slight sediment. 

 In Beer-wort, a thick sediment without film formation on the surface. 



