APPENDIX 



KISCHENSKY'S QUICK METHOD OF STAINING BACTERIA 

 IN COVER-GLASS AND SLIDE PREPARATIONS. 



1. A drop of a weak solution of carbol fuchsin (10 drops to 10 c.c. of 

 water) is placed on a cover-glass and mixed with a minimum quantity of 

 the pure culture under investigation, and spread out in a thin layer. 



2. The preparation is now heated gently over the flame. 



3. In examining blood, pus, etc., the above stain is mixed with an 

 alcoholic solution of methylene blue. 



The results are : the quickness of the method, beautiful staining, by 

 which means the flagella can also be demonstrated, while the field 

 remains unstained. 



SEMENOWICZ AND MARZINOWSKY'S SPECIAL METHOD OF 

 STAINING BACTERIA IN COVER-GLASS SPECIMENS AND 

 SECTIONS. 



1. The cover-glass specimens and sections are stained two (three to 

 four) minutes in a solution of carbol fuchsin (one part of a concentrated 

 solution and two parts of water). 



2. The specimens are now washed with water. 



3. Stain three to four (four to five) minutes with Loffler's methylene 

 blue. 



4. Wash in alcohol. 



5. Clarify in oil and xylol. 



Results. Loffler's blue displaces the carbol fuchsin in the bacteria 

 and appears to work on the principle of a mordant. The nuclei and 

 the bacteria are stained blue, the interstitial tissue and the protoplasm 

 of the cells red, while degenerated bacteria also appear red. 



GASTROMYCOSIS OVIS (NIELSEN). 



This organism was isolated in a very rapidly fatal endemic disease 

 amongst sheep in Iceland and Norway, characterised by a haemorrhagic 



