272 APPENDIX 



inflammation of the abomasum and the other compartments of the 

 stomach, portions of the intestines, as well as the whole body. The 

 bacilli were found in the walls of the stomach and frequently in the 

 internal organs three hours after death. This disease, from the 

 description given by Nielsen, is probably identical with a disease 

 affecting sheep in the country known as ' Braxy.' 



Microscopical Appearances. Bacilli from 1 to 2 by 6 p, often 

 occurring in pairs, seldom arranged in chains, frequently containing 

 refractile bodies. 



Staining Reactions. Stains by the Gram method. 



The bacillus has not been cultivated on artificial media. Experiments 

 conducted with pieces of tissue containing the bacilli, introduced into 

 rabbits and lambs, as well as feeding experiments, yielded negative 

 results. 



SPECIAL BACILLUS ISOLATED FROM MILK IN 

 PIORKOWSKI'S LABORATORY, BY CAMPBELL M'CLURE 



(Deutsche Med. Wochemchrift, 1898, No. 26). 



Microscopical Appearances. It occurs as short rods a little shorter 

 and thicker than the diphtheria bacillus. Club-shapes are often observed, 

 especially in old cultures. 



Motility. Non-motile. 

 Spore Formation absent. 



Staining Reactions. Stains with any of the ordinary dyes, but not 

 by the Gram method. 



Biological Characters. On Gelatine Plates. In two to three days 

 a luxuriant growth develops on the surface of the medium of small white, 

 slightly transparent, granular -points, with well-defined borders, becoming 

 later (in forty-eight hours) yellowish-brown in colour, dark in the middle, 

 and clear at the periphery. 



In Gelatine Slab Cultures opaque white points develop along the 

 inoculation track, tending to become confluent in the centre. 



On Agar Plates at 37 C., light brown granular colonies develop. 



On Glycerine Agar, a similar growth. 



Bouillon at 37 C. becomes cloudy, a flaky sediment forming on the 

 bottom and sides of the tube. 



On Potatoes, at 37 C. a copious, greyish-white, moist coating, with 

 dentated edges, which become yellow in older cultures. 



On Grape Sugar Agar Media the growth exhibits no special 

 characteristics. 



Milk inoculated and kept at 37 C. for forty-eight hours has an acid 



