PREFACE 



THIS manual is the outgrowth of a course in biochemical 

 methods given by the undersigned in the Yale School of Medicine. 

 Requests for copies of the mimeographed manual have been so 

 numerous that it has seemed desirable to print it in book form. 

 With this point in view several methods not originally in the 

 course have been included in order to give the manual a more 

 general application. It is believed that the methods herein 

 described will meet not only the needs of medical schools desiring 

 a fundamental course in biochemical methods, but will also be 

 useful for physicians, research and commercial laboratories, and 

 hospitals. 



No claim of originality is made for any of the methods. They 

 have been compiled from such sources as the Journal of Biological 

 Chemistry, the American Journal of Physiology, Journal of 

 Physiology, etc., various text books, such as Hawk's Practical 

 Physiological Chemistry, Macleod's Physiology and Biochemistry 

 in Modern Medicine, Folin's Manual of Biological Chemistry, 

 Treadwell-Hall Analytical Chemistry, Fresenius-Cohn Quantita- 

 tive Chemical Analysis, etc. 



To Dr. Michael Ringer I am indebted for aid in selecting and 

 testing methods and to Miss M. Eloise Montague my thanks are 

 due for preparation of the manuscript and proof reading. 



FRANK P. UNDERBILL. 

 NEW HAVEN, 

 July 1, 1921. 



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