PART III 

 METHODS FOR THE ANALYSIS OF BLOOD 



TOTAL SOLIDS 



IF total solid determination only is desired, approximately 0.5 

 to 1.0 gm. of blood is drawn into weighed aluminum dishes with 

 tightly fitting covers. After a second weighing the covers are 

 removed and the blood is dried to constant weight in an oven at 

 a temperature of 105 to 110 C. Usually drying for a period of 

 three hours is sufficient. Place in desiccator for one-half hour. 

 Weigh. If it is desired to make a determination of the ash on the 

 same sample the blood should be drawn in small porcelain crucibles. 



ASH DETERMINATION 



The dried, weighed blood is heated in a weighed porcelain 

 crucible over a low flame until the ash is white on cooling. The 

 temperature should be such that the bottom of the crucible shows 

 only a dull red glow. Heating too rapidly will cause a loss of ash 

 owing to volatilization of the chlorides. After the ashing is 

 complete place crucible in desiccator for one-half hour. Weigh. 



BLOOD ANALYSIS 



System of Folin and Wu l 



For the estimation of Non-Protein Nitrogen, Urea, Creatinine, 

 Creatine, Uric Acid and Sugar. 



In this system of blood analysis for the above subjects a 

 sufficiently large quantity of blood, namely, 10 c.c. or more, is 



1 Folin and Wu: Jour. Biol. Chem., 1919, 38, 81. 

 83 



