282 



METHODS OF ANALYSIS 



[Chap. 



AMINO NITROGEN. 

 Van Slyke Method^''. — Tentative. 



44 REAGENTS. 



(a) Alkaline ■permanganate solution. — Dissolve 50 grams of potassium permanga- 

 nate and 25 grams of potassium hj'droxid in sufficient water to make 1 liter. 



(b) Sodium nitrite solution. — Dissolve 30 grams of sodium nitrite in sufficient 

 water to make 100 cc. 



(C) Glacial acetic acid. 



FIG. 10. VAN SLYKE APPARATUS FOR THE DETERMINATION OF AMINO NITROGEN. 



{By courtesy of the Journal of Biological Chemistry.) 



45 APPARATUS. 



Employ the apparatus shown in Figs. 10 and 11, the former illustrating the man- 

 ner in which the entire apparatus is arranged and the latter showing the details 



