XXI] 



MEAT AND MEAT PRODUCTS 



283 



of the deaminizing bulb and connections, 

 the alkaline permanganate solution. 



The Hempel gas pipette is filled with 



46 DETERMINATION. 



Fill with water the burette (F), the capillary tube leading to the Hempel pipette 

 and also the other capillary as far as c. Introduce into A sufficient glacial acetic 

 acid to fill one fifth of D, the tube (A) being etched with a mark to measure this 



P'pette 



FIG. 11. DETAILS OF THE DEAMINIZING BULB AND CONNECTION. 



(By courtesy of the Journal of Biological Chemistry.) 



amount. Allow the acid to run into D, the cock c being turned so as to allow the 

 air to escape from D. Pour the sodium nitrite solution into A until D is full of solu- 

 tion and enough e.xcess is present to rise a little above the cock into A. A is also 

 marked for measuring off this amount. Then close the gas exit from D at c, and, 

 a being open, shake D for a few seconds until the liquid is forced down to the 20 

 cc. mark in D. Then close a, open c and shake the apparatus rapidly with the 

 motor for 2 minutes, these operations being for the purpose of expelling all the air 

 from D. Then turn c and / so that D and F are connected. 



